{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T21:21:30Z","timestamp":1776288090753,"version":"3.50.1"},"reference-count":64,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T00:00:00Z","timestamp":1717545600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,5]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> The fungal pathogen <jats:italic>Aspergillus fumigatus<\/jats:italic> can induce prolonged colonization of the lungs of susceptible patients, resulting in conditions such as allergic bronchopulmonary aspergillosis and chronic pulmonary aspergillosis.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold> Analysis of the <jats:italic>A. fumigatus<\/jats:italic> secretome released during sub-lethal infection of <jats:italic>G. mellonella<\/jats:italic> larvae may give an insight into products released during prolonged human colonisation.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold>\n                  <jats:italic>Galleria mellonella<\/jats:italic> larvae were infected with <jats:italic>A. fumigatus,<\/jats:italic> and the metabolism of host carbohydrate and proteins and production of fungal virulence factors were analysed. Label-free qualitative proteomic analysis was performed to identify fungal proteins in larvae at 96\u2009hours post-infection and also to identify changes in the <jats:italic>Galleria<\/jats:italic> proteome as a result of infection.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Infected larvae demonstrated increasing concentrations of gliotoxin and siderophore and displayed reduced amounts of haemolymph carbohydrate and protein. Fungal proteins (399) were detected by qualitative proteomic analysis in cell-free haemolymph at 96\u2009hours and could be categorized into seven groups, including virulence (<jats:italic>n<\/jats:italic> = 25), stress response (<jats:italic>n<\/jats:italic> = 34), DNA repair and replication (<jats:italic>n<\/jats:italic> = 39), translation (<jats:italic>n<\/jats:italic> = 22), metabolism (<jats:italic>n<\/jats:italic> = 42), released intracellular (<jats:italic>n<\/jats:italic> = 28) and cellular development and cell cycle (<jats:italic>n<\/jats:italic> = 53). Analysis of the Gallerial proteome at 96\u2009hours post-infection revealed changes in the abundance of proteins associated with immune function, metabolism, cellular structure, insect development, transcription\/translation and detoxification.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Characterizing the impact of the fungal secretome on the host may provide an insight into how <jats:italic>A. fumigatus<\/jats:italic> damages tissue and suppresses the immune response during long-term pulmonary colonization.<\/jats:p>","DOI":"10.1099\/jmm.0.001844","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T13:17:32Z","timestamp":1717593452000},"source":"Crossref","is-referenced-by-count":2,"title":["Characterization of Aspergillus fumigatus secretome during sublethal infection of Galleria mellonella larvae"],"prefix":"10.1099","volume":"73","author":[{"given":"Aaron","family":"Curtis","sequence":"first","affiliation":[{"name":"Department of Biology, Maynooth University, Maynooth, Co. 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