{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:20:09Z","timestamp":1777389609450,"version":"3.51.4"},"reference-count":26,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"Beijing Public Health Experts Project","award":["2022-3-040"],"award-info":[{"award-number":["2022-3-040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,8]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Aminoglycoside antibiotics such as amikacin and kanamycin are important components in the treatment of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (Mtb) infection. However, more and more clinical strains are found to be aminoglycoside antibiotic-resistant. Apramycin is another kind of aminoglycoside antibiotic that is commonly used to treat infections in animals.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold> Apramycin may have <jats:italic>in vitro<\/jats:italic> activity against Mtb.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> This study aims to evaluate the efficacy of apramycin against Mtb <jats:italic>in vitro<\/jats:italic> and determine its epidemiological cut-off (ECOFF) value.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> One hundred Mtb isolates, including 17 pansusceptible and 83 drug-resistant tuberculosis (DR-TB) strains, were analysed for apramycin resistance using the MIC assay.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Apramycin exhibited significant inhibitory activity against Mtb clinical isolates, with an MIC<jats:sub>50<\/jats:sub> of 0.5 \u03bcg ml<jats:sup>\u22121<\/jats:sup> and an MIC<jats:sub>90<\/jats:sub> of 1 \u03bcg ml<jats:sup>\u22121<\/jats:sup>. We determined the tentative ECOFF value as 1\u2009\u00b5g\u2009ml<jats:sup>\u22121<\/jats:sup> for apramycin. The resistant rates of multidrug-resistant tuberculosis (MDR-TB), pre-extensively drug-resistant (pre-XDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) strains were 12.12\u200a% (4\/33), 20.69\u200a% (6\/29) and 66.67\u200a% (14\/21), respectively. The <jats:italic>rrs<\/jats:italic> gene A1401G is associated with apramycin resistance, as well as the cross-resistance between apramycin and other aminoglycosides.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Apramycin shows high <jats:italic>in vitro<\/jats:italic> activity against the Mtb clinical isolates, especially the MDR-TB clinical isolates. This encouraging discovery calls for more research on the functions of apramycin <jats:italic>in vivo<\/jats:italic> and as a possible antibiotic for the treatment of drug-resistant TB.<\/jats:p>","DOI":"10.1099\/jmm.0.001854","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T10:18:36Z","timestamp":1720433916000},"source":"Crossref","is-referenced-by-count":5,"title":["Apramycin has high in vitro activity against Mycobacterium tuberculosis"],"prefix":"10.1099","volume":"73","author":[{"given":"Qing","family":"Sun","sequence":"first","affiliation":[{"name":"National Clinical Laboratory on Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing Chest Hospital, Capital Medical University, Beijing, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing 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