{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T21:29:39Z","timestamp":1753738179308,"version":"3.40.5"},"reference-count":39,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82272352"],"award-info":[{"award-number":["82272352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,16]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold>\n                  <jats:italic>Klebsiella<\/jats:italic> spp. are important bacteria that colonize the human intestine, especially in preterm infants; they can induce local and systemic disease under specific circumstances, including inflammatory bowel disease, necrotizing enterocolitis and colorectal cancer.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold>\n                  <jats:italic>Klebsiella<\/jats:italic> spp. colonized in the intestine of the neonates in the neonatal intensive care unit (NICU) may be associated with disease and antibiotic resistance, which will be hazardous to the children.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> Our aim was to know about the prevalence, antimicrobial resistance and genome characteristics of <jats:italic>Klebsiella<\/jats:italic> spp. in neonate carriers.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Genome sequencing and analysis, and antimicrobial susceptibility testing were mainly performed in this study.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> The isolation rates of <jats:italic>Klebsiella<\/jats:italic> spp. strains were 3.7% (16\/436) in 2014 and 4.3% (18\/420) in 2021. Cases with intestinal-colonized <jats:italic>Klebsiella<\/jats:italic> spp. were mainly infants with low birth weights or those with pneumonia or hyperbilirubinemia. According to the core-pan genomic analysis, 34 stains showed gene polymorphism and a sequence type (ST) of an emerging high-risk clone (ST11). Eight strains (23.5%) were found to be resistant to 2 or more antibiotics, and 46 genes\/gene families along with nine plasmids were identified that conferred resistance to antibiotics. In particular, the two strains were multidrug-resistant. Strain A1256 that is related to <jats:italic>Klebsiella quasipneumoniae subsp. similipneumoniae<\/jats:italic> was uncommon, carrying two plasmids similar to IncFII and IncX3 that included five antibiotic resistance genes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> The prevention and control of neonatal <jats:italic>Klebsiella<\/jats:italic> spp. colonization in the NICU should be strengthened by paying increased attention to preventing antimicrobial resistance in neonates.<\/jats:p>","DOI":"10.1099\/jmm.0.001862","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T15:17:20Z","timestamp":1723821440000},"source":"Crossref","is-referenced-by-count":2,"title":["Antimicrobial resistance profiles and genome characteristics of Klebsiella isolated from the faeces of neonates in the neonatal intensive care 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