{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:30:15Z","timestamp":1768260615500,"version":"3.49.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"8","funder":[{"name":"the Doctoral Scientific Research Foundation of the First Affiliated Hospital of Kunming Medical University","award":["Grant No.2022BS020"],"award-info":[{"award-number":["Grant No.2022BS020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,22]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction<\/jats:bold>. Cytotoxin-associated gene A (CagA) from <jats:italic>Helicobacter pylori<\/jats:italic> is highly related to chronic gastritis. Tyrosine phosphorylation of Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs from CagA determines the pathogenicity of <jats:italic>H. pylori<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap statement<\/jats:bold>. The precise amino acid variations surrounding the EPIYA motifs and their correlation with clinical outcomes have been poorly explored.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold>. The purpose of this study was to examine the CagA 3\u2032 region polymorphism of <jats:italic>H. pylori<\/jats:italic> and its association with chronic gastritis in the Chinese population.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Method<\/jats:bold>. A total of 86 <jats:italic>cagA<\/jats:italic>-positive <jats:italic>H. pylori<\/jats:italic> strains were isolated from patients with chronic gastritis in two different hospitals in Beijing, PR China. Genomic DNA was extracted commercial kits, and the <jats:italic>cagA<\/jats:italic> 3\u2032 variable region of <jats:italic>H. pylori<\/jats:italic> was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analysed using the CLC Sequence Viewer, BioEdit, and WebLogo 3.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results<\/jats:bold>. Two hundred and fifty-nine EPIYA motifs were identified from <jats:italic>cagA<\/jats:italic>-positive <jats:italic>H. pylori<\/jats:italic> strains. Notably, EPIYA-B exhibited a higher frequency of variation in comparison to EPIYA-A, EPIYA-C, and EPIYA-D. The prevalent sequences for East-Asian-type CagA were QVNK and TIDF, while KVNK and TIDD were most commonly observed for Western-type CagA. The CRPIA motifs of East-Asian-type CagA and Western-type CagA varied at positions 4, 6, 7, 8, and 10. CagA-ABD (73.2%) was the most prevalent type, followed by CagA-ABC (18.6%) and CagA-AB (3.4%). The ratio of CagA-ABD was observed to be higher in cases of chronic non-atrophic gastritis with erosive (NAGE) or chronic atrophic gastritis (AG) compared to chronic non-atrophic gastritis (NAG), and the difference was found to be statistically significant (\u03c72=59.000\/64.000, <jats:italic>P<\/jats:italic>&lt;0.001).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions<\/jats:bold>. The EPIYA segments of Western-type CagA and East-Asian-type CagA differ significantly and the presence of CagA-ABD may be associated with severe chronic gastritis from this study.<\/jats:p>","DOI":"10.1099\/jmm.0.001880","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T11:17:33Z","timestamp":1724325453000},"source":"Crossref","is-referenced-by-count":3,"title":["CagA 3\u2032 region polymorphism of Helicobacter pylori and its association with chronic gastritis in the Chinese population"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-3412","authenticated-orcid":false,"given":"Xiaoyan","family":"Zhu","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Laboratory Medicine, Kunming, PR China"},{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, PR China"},{"name":"Yunnan Province Clinical Research Center for Laboratory Medicine, 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