{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T01:17:27Z","timestamp":1771982247897,"version":"3.50.1"},"reference-count":40,"publisher":"Microbiology Society","issue":"9","funder":[{"name":"Institutional funding","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,25]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold>\n                  <jats:italic>Tropheryma whipplei<\/jats:italic> is responsible for the classical Whipple\u2019s disease. Recently, a new <jats:italic>Tropheryma<\/jats:italic> species was described in a Belgian immunocompromised patient with pleuritis.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap Statement.<\/jats:bold> There is currently no specific molecular diagnostic test detecting other <jats:italic>Tropheryma<\/jats:italic> species than <jats:italic>Tropheryma whipplei<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To develop and validate two broad-range pan-<jats:italic>Tropheryma<\/jats:italic> genus PCRs detecting both <jats:italic>T. whipplei<\/jats:italic> and new <jats:italic>Tropheryma<\/jats:italic> species.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> From shotgun sequencing data of the lung tissue biopsy of the Belgian subject, we designed two PCRs targeting the 23S rRNA and <jats:italic>rnpB<\/jats:italic> genes. Prospectively, requests for <jats:italic>T. whipplei<\/jats:italic> PCR were tested with <jats:italic>T. whipplei<\/jats:italic>-specific PCRs and the two <jats:italic>Tropheryma<\/jats:italic> broad-range PCRs from January 2019 to November 2022.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> In total, 2605\u2009samples were tested using both the pan-<jats:italic>Tropheryma<\/jats:italic> 23S rRNA PCR and the <jats:italic>T. whipplei<\/jats:italic>-specific PCR. In addition, 833 of the 2605\u2009samples were also tested using the pan-<jats:italic>Tropheryma rnpB<\/jats:italic> PCRs. Sensitivity was 78.8% and 79.7% for 23S rRNA and <jats:italic>rnpB<\/jats:italic> PCRs, as compared with the species-specific <jats:italic>T. whipplei<\/jats:italic> PCR. Specificity was 99.9% and 99.7% for the 23S rRNA and the <jats:italic>rnpB<\/jats:italic> PCRs, respectively. We identified a patient whose bronchoalveolar lavage tested positive with the two broad-range PCRs with &gt;10<jats:sup>5<\/jats:sup> copies ml<jats:sup>\u22121<\/jats:sup>. Specific <jats:italic>T. whipplei<\/jats:italic> PCRs were negative. Known for panuveitis, this 49-year-old male presented with an eye inflammation recurrence, and a CT scan showed multiple mediastino-hilar necrotic adenopathies. Doxycyclin (1\u2009year), hydroxychloroquin (1\u2009year) and co-trimoxazol (1\u2009month) treatments led to a favourable outcome.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Specific <jats:italic>T. whipplei<\/jats:italic> PCR exhibited better sensitivity than the pan-<jats:italic>Tropheryma<\/jats:italic> PCRs. However, both broad-range pan-<jats:italic>Tropheryma<\/jats:italic> PCRs demonstrated excellent specificity and were pivotal to identifying a new probable case of <jats:italic>Tropheryma<\/jats:italic> infection due to another species-level lineage.<\/jats:p>","DOI":"10.1099\/jmm.0.001889","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T15:12:37Z","timestamp":1727277157000},"source":"Crossref","is-referenced-by-count":2,"title":["Development of novel broad-range pan-genus PCR assays for the detection of Tropheryma species"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3664-2139","authenticated-orcid":false,"given":"Florian","family":"Tagini","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Institute of Microbiology, Lausanne University Hospital and University of Lausanne, Lausanne, 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