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The MIC and antibiofilm activity were determined using broth microdilution. Checkerboard and time-kill assays evaluated the combination of O.geo and polymyxin B (PMB), whereas a protein leakage assay verified its action.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Eugenol (39.67%) was a major constituent identified. The MIC of the O.geo alone ranged from 128 to 512 \u00b5g ml<jats:sup>\u22121<\/jats:sup>. The fractional inhibitory concentration index (0.28) and time-kill assay showed a synergism. In addition, O.geo and PMB inhibited biofilm formation and increased protein leakage in the plasma membrane. The treatment was tested <jats:italic>in vivo<\/jats:italic> using a <jats:italic>Caenorhabditis elegans<\/jats:italic> model, and significantly increased survival without toxicity was observed.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> O.geo could be used as a potential therapeutic alternative to combat infections caused by multidrug-resistant bacteria, especially in combination with PMB.<\/jats:p>","DOI":"10.1099\/jmm.0.001891","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T15:16:35Z","timestamp":1726672595000},"source":"Crossref","is-referenced-by-count":2,"title":["Polymyxin combined with Ocimum gratissimum essential oil: one alternative strategy for combating polymyxin-resistant Klebsiella pneumoniae"],"prefix":"10.1099","volume":"73","author":[{"given":"Andressa Leite Ferraz","family":"de Melo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Ci\u00eancias da 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