{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:45:24Z","timestamp":1790840724634,"version":"4.1.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ningbo Medical and Health Brand Discipline","award":["PPXK2024-06"],"award-info":[{"award-number":["PPXK2024-06"]}]},{"name":"Ningbo Clinical Research Center for Children\u2019s Health and Diseases","award":["no. 2019A21002"],"award-info":[{"award-number":["no. 2019A21002"]}]},{"name":"the Ningbo key discipline Pediatrics","award":["no. 2022-B17"],"award-info":[{"award-number":["no. 2022-B17"]}]},{"name":"the Innovation Project of Distinguished Medical Team in Ningbo","award":["no. 2022020405"],"award-info":[{"award-number":["no. 2022020405"]}]},{"name":"the Ningbo public welfare project","award":["no. 2022S127"],"award-info":[{"award-number":["no. 2022S127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction.<\/jats:bold>\n                    Rotavirus A is the most common pathogen causing diarrhoea in children less than 5\u2009years, leading to severe complications such as dehydration, electrolyte imbalances, acidosis, myocarditis, convulsions, pneumonia, and other life-threatening conditions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Gap statement.<\/jats:bold>\n                    There is an urgent need for a rapid and efficient nucleic acid detection strategy to enable early diagnosis and treatment, preventing rotavirus transmission and associated complications.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    This article aimed to develop a nuclear acid sequence-based amplification (NASBA)-Cas12a system for detecting rotavirus A using fluorescence intensity or lateral flow strips.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methodology.<\/jats:bold>\n                    The NASBA technology was combined with the clustered regularly interspaced short palindromic repeats-Cas12a system to establish a NASBA-Cas12a system for detecting rotavirus A.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    The NASBA-Cas12a system could detect rotavirus A at 37 \u2103 within 70\u2009min and had no cross-reactivity with other viruses, achieving a limit of detection of 1.2 copies \u03bcl\n                    <jats:sup>\u20131<\/jats:sup>\n                    . This system demonstrated a sensitivity of 100%, specificity of 90%, positive predictive value of 97.22% and negative predictive value of 100%. The kappa value was 0.933, indicating that the NASBA-Cas12a system was highly consistent with reverse transcription-PCR.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    The NASBA-Cas12a system exhibited high sensitivity and specificity for detecting rotavirus A, showing great potential for clinical application.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.001892","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T09:18:16Z","timestamp":1727947096000},"source":"Crossref","is-referenced-by-count":11,"title":["A rapid visualization method for detecting rotavirus A by combining nuclear acid sequence-based amplification with the CRISPR-Cas12a assay"],"prefix":"10.1099","volume":"73","author":[{"given":"Yue","family":"Chen","sequence":"first","affiliation":[{"name":"Health Science Center, Ningbo University, Ningbo, Zhejiang 315000, PR 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