{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:09:59Z","timestamp":1747181399367,"version":"3.40.5"},"reference-count":71,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,26]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Disruptions in gut microbiota, known as dysbiosis, have been increasingly linked to pathogenic infections, with <jats:italic>Salmonella<\/jats:italic> Typhimurium being a notable contributor to these disturbances.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold> We hypothesize that the <jats:italic>S<\/jats:italic>. Typhimurium 14028 WT strain induces significant dysbiosis in the rat gut microbiota and that the <jats:italic>dam<\/jats:italic> and <jats:italic>seqA<\/jats:italic> genes play crucial roles in this process.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold>. In this study, it was aimed at investigating the dysbiotic activity of the <jats:italic>S<\/jats:italic>. Typhimurium 14028 WT strain on the rat gut microbiota and the roles of <jats:italic>dam<\/jats:italic> and <jats:italic>seqA<\/jats:italic> genes on this activity.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Method.<\/jats:bold> Changes in the rat gut microbiota were determined by examining the anal swap samples taken from the experimental groups of these animals using 16S rRNA high-throughput sequencing technology.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> In the experimental groups, the dominant phyla were determined to be <jats:italic>Firmicutes<\/jats:italic> and <jats:italic>Bacteroidetes<\/jats:italic> (<jats:italic>P<\/jats:italic>&lt;0.05). However, while the rate of <jats:italic>Bacteroidetes<\/jats:italic> was significantly reduced in those treated with the WT and <jats:italic>seqA<\/jats:italic> mutants, no significant difference was observed in the <jats:italic>dam<\/jats:italic> mutant compared to the control group (<jats:italic>P<\/jats:italic>&lt;0.05). In all experimental animals, the dominant species was determined to be <jats:italic>Prevotella copri<\/jats:italic>, regardless of the experiment time and application. The analysis results of the samples taken on the third day from the rat groups infected with the <jats:italic>S.<\/jats:italic> Typhimurium 14028 WT strain (W2) presented the most striking data of this study.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Through distance analysis, we demonstrated that a successful <jats:italic>Salmonella<\/jats:italic> infection completely changes the composition of the microbiota, dramatically reduces species diversity and richness in the microbiota and encourages the growth of opportunistic pathogens.<\/jats:p>","DOI":"10.1099\/jmm.0.001893","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T11:16:30Z","timestamp":1727435790000},"source":"Crossref","is-referenced-by-count":0,"title":["Relationship of Salmonella Typhimurium 14028 strain and its dam and seqA mutants with gut microbiota dysbiosis in 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