{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:29Z","timestamp":1787010569086,"version":"build-2736575974"},"reference-count":48,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Seed funding for new staff"},{"name":"General Research Fund","award":["17121820"],"award-info":[{"award-number":["17121820"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,8]]},"abstract":"<jats:p>\n                    <jats:bold>Background.<\/jats:bold>\n                    Bacteria significantly influence human health and disease, with bacterial biosynthetic gene clusters (BGCs) being crucial in the microbiome\u2013host and microbe\u2013microbe interactions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Gap statement.<\/jats:bold>\n                    Despite extensive research into BGCs within the human gut microbiome, their roles in the oral microbiome are less understood.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    This pilot study utilizes high-throughput shotgun metagenomic sequencing to examine the oral microbiota in different niches, particularly focusing on the association of BGCs with periodontitis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methodology.<\/jats:bold>\n                    We analysed saliva, subgingival plaque and supragingival plaque samples from periodontitis patients (\n                    <jats:italic>n<\/jats:italic>\n                    =23) and controls (\n                    <jats:italic>n<\/jats:italic>\n                    =16). DNA was extracted from these samples using standardized protocols. The high-throughput shotgun metagenomic sequencing was then performed to obtain comprehensive genetic information from the microbial communities present in the samples.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    Our study identified 10\u2009742 BGCs, with certain clusters being niche-specific. Notably, aryl polyenes and bacteriocins were the most prevalent BGCs identified. We discovered several \u2018novel\u2019 BGCs that are widely represented across various bacterial phyla and identified BGCs that had different distributions between periodontitis and control subjects. Our systematic approach unveiled the previously unexplored biosynthetic pathways that may be key players in periodontitis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions.<\/jats:bold>\n                    Our research expands the current metagenomic knowledge of the oral microbiota in both healthy and periodontally diseased states. These findings highlight the presence of novel biosynthetic pathways in the oral cavity and suggest a complex network of host\u2013microbe and microbe\u2013microbe interactions, potentially influencing periodontal disease. The BGCs identified in this study pave the way for future investigations into the role of small-molecule-mediated interactions within the human oral microbiota and their impact on periodontitis.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.001898","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T12:17:48Z","timestamp":1728389868000},"source":"Crossref","is-referenced-by-count":6,"title":["Multi-site analysis of biosynthetic gene clusters from the periodontitis oral microbiome"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7286-0427","authenticated-orcid":false,"given":"Mohamad","family":"Koohi-Moghadam","sequence":"first","affiliation":[{"name":"Department of Diagnostic Radiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong, PR China"},{"name":"Division of Applied Oral Sciences & Community Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rory M.","family":"Watt","sequence":"additional","affiliation":[{"name":"Division of Applied Oral Sciences & Community Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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