{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:02:43Z","timestamp":1789513363630,"version":"build-2803163510"},"reference-count":38,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"Consejo Nacional de Humanidades Ciencias y Tecnolog\u00edas CONAHCYT","award":["Ciencia de Frontera CF-2019- 610281"],"award-info":[{"award-number":["Ciencia de Frontera CF-2019- 610281"]}]},{"name":"Consejo Nacional de Humanidades Ciencias y Tecnolog\u00edas CONAHCYT","award":["Fellowship No. 765891"],"award-info":[{"award-number":["Fellowship No. 765891"]}]},{"name":"Consejo Nacional de Humanidades Ciencias y Tecnolog\u00edas CONAHCYT","award":["Fellowship No. 623207"],"award-info":[{"award-number":["Fellowship No. 623207"]}]},{"name":"Consejo Nacional de Humanidades Ciencias y Tecnolog\u00edas CONAHCYT","award":["104640"],"award-info":[{"award-number":["104640"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction<\/jats:bold>. Adherence is a major virulence trait in <jats:italic>Candida glabrata<\/jats:italic> that, in many strains, depends on the <jats:italic>EPA<\/jats:italic> (epithelial adhesin) genes, which confer the ability to adhere to epithelial and endothelial cells of the host. The <jats:italic>EPA<\/jats:italic> genes are generally found at subtelomeric regions, which makes them subject to subtelomeric silencing. In <jats:italic>C. glabrata<\/jats:italic>, subtelomeric silencing depends on different protein complexes, such as silent information regulator and yKu complexes, and other proteins, such as Repressor\/activator protein 1 (Rap1) and Abf1. At the <jats:italic>EPA1<\/jats:italic> locus, which encodes the main adhesin Epa1, we previously found at least two <jats:italic>cis<\/jats:italic>-acting elements, the protosilencer Sil2126 and the negative element, that contribute to the propagation of silencing from the telomere to the subtelomeric region.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis<\/jats:bold>. Abf1 binds to the regulatory regions of <jats:italic>EPA1<\/jats:italic> and other regions at the telomere E-R, thereby negatively regulating <jats:italic>EPA1<\/jats:italic> transcription.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold>. To determine whether Abf1 and Rap1 silencing proteins bind to previously identified <jats:italic>cis-<\/jats:italic>acting elements on the right telomere of chromosome E (E-R subtelomeric region), resulting in negative regulation of <jats:italic>EPA1<\/jats:italic> transcription and infer Abf1 and Rap1 recognition sites in <jats:italic>C. glabrata<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology<\/jats:bold>. We used chromatin immunoprecipitation (ChIP) followed by quantitative PCR to determine the binding sites for Abf1 and Rap1 in the intergenic regions between <jats:italic>EPA1<\/jats:italic> and <jats:italic>EPA2<\/jats:italic> and <jats:italic>HYR1<\/jats:italic> and <jats:italic>EPA1<\/jats:italic>, and mutants were used to determine the silencing level of the <jats:italic>EPA1<\/jats:italic> promoter region.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results<\/jats:bold>. We found that Abf1 predominantly binds to the <jats:italic>EPA1<\/jats:italic> promoter region, leading to negative regulation of <jats:italic>EPA1<\/jats:italic> expression. Furthermore, the mutant <jats:italic>abf1-43<\/jats:italic>, which lacks the last 43 amino acids at its C-terminal end and is defective for subtelomeric silencing, exhibits hyperadherence to epithelial cells <jats:italic>in vitro<\/jats:italic> compared to the parental strain, suggesting that <jats:italic>EPA1<\/jats:italic> is derepressed. We also determined the motif-binding sequences for Abf1 and Rap1 in <jats:italic>C. glabrata<\/jats:italic> using data from the ChIP assays.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion<\/jats:bold>. Together these data indicate that Abf1 negatively regulates <jats:italic>EPA1<\/jats:italic> expression, leading to decreased adhesion of <jats:italic>C. glabrata<\/jats:italic> to epithelial cells.<\/jats:p>","DOI":"10.1099\/jmm.0.001905","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T13:18:32Z","timestamp":1727961512000},"source":"Crossref","is-referenced-by-count":2,"title":["Abf1 negatively regulates the expression of EPA1 and affects adhesion in Candida glabrata"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0800-0529","authenticated-orcid":false,"given":"Grecia","family":"Hern\u00e1ndez-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Divisi\u00f3n de Biolog\u00eda Molecular. IPICYT. Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Camino a la Presa San Jos\u00e9, #2055, Col. Lomas 4\u00aa secci\u00f3n. San Luis Potos\u00ed, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2224-8109","authenticated-orcid":false,"given":"Laura A.","family":"Vera-Salazar","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Biolog\u00eda Molecular. IPICYT. Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Camino a la Presa San Jos\u00e9, #2055, Col. Lomas 4\u00aa secci\u00f3n. San Luis Potos\u00ed, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7204-5142","authenticated-orcid":false,"given":"Guadalupe","family":"Guti\u00e9rrez-Escobedo","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Biolog\u00eda Molecular. IPICYT. Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Camino a la Presa San Jos\u00e9, #2055, Col. Lomas 4\u00aa secci\u00f3n. San Luis Potos\u00ed, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-5258","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"G\u00f3mez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Biolog\u00eda Molecular. IPICYT. Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Camino a la Presa San Jos\u00e9, #2055, Col. Lomas 4\u00aa secci\u00f3n. San Luis Potos\u00ed, San Luis Potos\u00ed 78216, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6718-0005","authenticated-orcid":false,"given":"Osney","family":"Leiva-Pel\u00e1ez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Biolog\u00eda Molecular. IPICYT. Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Camino a la Presa San Jos\u00e9, #2055, Col. Lomas 4\u00aa secci\u00f3n. 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