{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:25:47Z","timestamp":1790508347665,"version":"4.1.0"},"reference-count":26,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"\n Introduction.<\/jats:bold>\n Laboratory participation in external quality assessment (EQA) programmes including proficiency testing (PT) is a requirement of clinical laboratory conformance to ISO 15189:2022\n Medical laboratories \u2013 Requirements for quality and competence<\/jats:italic>\n . PT is one EQA method whereby laboratories are sent blinded samples for characterization by routine laboratory diagnostic methods. Importantly, PT enables a laboratory\u2019s performance to be evaluated in comparison to the standard reference methods and to the performance of other peer laboratories using similar diagnostic methods.\n <\/jats:p>\n \n Gap statement.<\/jats:bold>\n The desired outcome of participating in PT is to help laboratories identify possible sources of error in each step of the total testing process and particularly in their test methods during the analytical phase.\n <\/jats:p>\n \n Aim.<\/jats:bold>\n This cross-sectional study investigated the impact of using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) compared to conventional phenotypic biochemical testing on laboratory performance in a clinical bacteriology PT scheme.\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n During a 6-year period from 2017-2022, the Canadian Microbiology Proficiency Testing implemented 112 PT challenges comprising 22 different sample types and included 61 different bacterial species. This was translated into 5883 graded test events for analysis. Multiple logistic regression techniques were employed to explore the association between the test method employed and laboratory performance. The sample type and aerobic classification of challenge organisms were included as confounding variables.\n <\/jats:p>\n \n Results.<\/jats:bold>\n Laboratories using MALDI-TOF MS performed significantly better in characterizing microorganisms than laboratories using phenotypic biochemical testing alone [odds ratio OR = 5.68, confidence interval (CI): 3.92, 8.22] regardless of the sample type and aerobic classification. Notably, our analysis identified a significant association between anaerobic organisms and laboratory performance (OR: 0.24,\u2009CI: 0.17\u20130.35), suggesting that culturing and identifying fastidious organisms remains a significant obstacle for many clinical microbiology laboratories.\n <\/jats:p>\n \n Conclusions.<\/jats:bold>\n Although no method is infallible and its performance will depend on the validation and quality assurance procedures, this finding may help the management in the decision for implementing MALDI-TOF MS in the microbiology laboratory. This study highlights the important role PT providers play in the objective assessment of laboratory performance and how it can provide evidence for quality improvement.\n <\/jats:p>","DOI":"10.1099\/jmm.0.001915","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T10:16:28Z","timestamp":1730196988000},"source":"Crossref","is-referenced-by-count":1,"title":["Identification methods as a factor affecting the performance of clinical microbiology laboratories participating in an external quality assessment program: a cross-sectional, retrospective analysis"],"prefix":"10.1099","volume":"73","author":[{"given":"Jennifer","family":"Wu","sequence":"first","affiliation":[{"name":"Canadian Microbiology Proficiency Testing Program (CMPT), Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Saiful","family":"Alam","sequence":"additional","affiliation":[{"name":"School of Population and Public Health, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0636-6698","authenticated-orcid":false,"given":"Veronica","family":"Restelli","sequence":"additional","affiliation":[{"name":"Canadian Microbiology Proficiency Testing Program (CMPT), Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2519-2593","authenticated-orcid":false,"given":"Selvarani","family":"Vimalanathan","sequence":"additional","affiliation":[{"name":"Canadian Microbiology Proficiency Testing Program (CMPT), Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2185-688X","authenticated-orcid":false,"given":"Lucy A.","family":"Perrone","sequence":"additional","affiliation":[{"name":"Canadian Microbiology Proficiency Testing Program (CMPT), Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2009.045"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.03.023"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3109\/00365510903307962"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(01)00518-6"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1086\/502214"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1921-1923.1997"},{"key":"R7","article-title":"Review of proficiency testing services for clinical laboratories in the United States - final report of a technical working group","author":"Peterson","year":"2008"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2013.08.007"},{"key":"R9","article-title":"ISO 15189:2022 Medical laboratories \u2014 Requirements for quality and competence"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.03.028"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.03.012"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines10111881"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00791"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.02.008"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-017-3046-0"},{"key":"R16","article-title":"EQA Programs \u2013 Grading Schemes | cmpt"},{"key":"R17","article-title":"CMPT Members Portal"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-818630-5.10038-7"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2072-1439.2014.02.15"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03107-15"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14124"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2016.01.014"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0207822"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2017-0170"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit278"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01619-9"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001915?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T12:18:03Z","timestamp":1761567483000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.001915"}},"subtitle":["<xhtml:span xmlns:xhtml=\"http:\/\/www.w3.org\/1999\/xhtml\" xml:lang=\"en\"><span style=\"color: rgb(33, 51, 104);\">This article is part of the&#160;<\/span><a href=\"https:\/\/www.microbiologyresearch.org\/content\/diagnosis-of-infectious-disease#\" rel=\"noopener noreferrer\" target=\"_blank\"><span style=\"color: rgb(33, 51, 104);\">Diagnosis of Infectious Diseases<\/span><\/a><span style=\"color: rgb(33, 51, 104);\">&#160;collection.<\/span>\n<\/xhtml:span>"],"short-title":[],"issued":{"date-parts":[[2024,10,3]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,3]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.001915","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,3]]}}}