{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:47:51Z","timestamp":1793148471467,"version":"4.5.0"},"reference-count":59,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","award":["29418"],"award-info":[{"award-number":["29418"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Thailand"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,18]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction<\/jats:bold>. Group B <jats:italic>Streptococcus<\/jats:italic> (GBS) is a multi-host pathogen causing pneumonia and meningitis in humans as well as streptococcal diseases in tilapia and mastitis in cattle. Thailand has experienced a significant increase in GBS infections that greatly impact health and economics.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap statement<\/jats:bold>. The antimicrobial resistance (AMR) and genotype data of GBS in Thailand are still limited and require further study.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim<\/jats:bold>. This study aimed to describe AMR profiles and molecular characteristics, especially antimicrobial resistance genes (ARGs) and virulence factor (VF) genes of GBS in Thailand.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology<\/jats:bold>. AMR profiles of 221 GBS isolates from humans, fish and freshwater were examined. Whole-genome sequencing of 41 representative isolates was used to investigate capsular genotypes and sequence types (STs), ARGs and VF genotypes.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results<\/jats:bold>. All GBS isolates were susceptible to penicillin; the majority (99.1%) showed resistance to tetracycline. In addition, the rates of resistance to clindamycin, erythromycin and levofloxacin were 22.6%, 20.4% and 2.3%, respectively; multidrug-resistant (MDR) isolates (TE-E-CM and TE-E-CM-LVX) were 19.5%. Among 41 representative isolates, the dominant types were capsular genotype III (63.4%) and ST283 (43.9%). ARGs associated with resistance to tetracycline (<jats:italic>tetM<\/jats:italic>, <jats:italic>tetO<\/jats:italic> and <jats:italic>tetS<\/jats:italic>), erythromycin (<jats:italic>ermB<\/jats:italic>, <jats:italic>ermA<\/jats:italic>, <jats:italic>mefA<\/jats:italic> and <jats:italic>msrD<\/jats:italic>) and clindamycin (<jats:italic>lsaC<\/jats:italic>, <jats:italic>lsaE<\/jats:italic> and <jats:italic>lnuB<\/jats:italic>) were identified. Additionally, point mutations responsible for levofloxacin resistance, S81L in GyrA, S79F\/Y in ParC and H221Y in ParE, were found. The MDR isolates belonged to various STs, predominantly clustering in capsular types III (60.0%) and Ib (30.0%). The MDR-hypervirulent ST17 and ST19 harboured multiple ARGs and mutations affecting quinolone resistance. Different VF gene patterns were found among hypervirulent STs (ST12, ST17, ST19 and ST283). Notably, a unique <jats:sc>nt<\/jats:sc> deletion [c.(1013_1020)delG] in <jats:italic>pilA<\/jats:italic> was found only in ST283.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion<\/jats:bold>. This study elucidated significant antimicrobial characteristics of a substantial number of GBS in Thailand. Moreover, the distribution of the hypervirulent ST283 and the genotypes of MDR-hypervirulent GBS were first described.<\/jats:p>","DOI":"10.1099\/jmm.0.001970","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T15:17:17Z","timestamp":1742311037000},"source":"Crossref","is-referenced-by-count":10,"title":["Predomination of hypervirulent ST283 and genetic diversity of levofloxacin resistance in multidrug-resistant, hypervirulent Streptococcus agalactiae in Thailand"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7350-4234","authenticated-orcid":false,"given":"Wajeeorn","family":"Ouancharee","sequence":"first","affiliation":[{"name":"Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, 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