{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T23:17:52Z","timestamp":1784157472113,"version":"3.55.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006182","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Esp\u00edrito Santo","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006182","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,6]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> While <jats:italic>Mycobacterium tuberculosis<\/jats:italic> cells in sputum in sputum have been studied extensively, little is known of their properties in exhaled aerosols.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis.<\/jats:bold> As differentially culturable tubercle bacteria (DCTB) are readily found in sputum, we hypothesized that DCTB might also be present in aerosols and potentially contribute to transmission.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To test cough aerosols from recently diagnosed pulmonary tuberculosis (TB) patients for DCTB.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> Cough-generated aerosols and sputum samples were collected from active pulmonary TB patients (<jats:italic>n<\/jats:italic>=27). A cough aerosol sampling system was modified to include both an Andersen Cascade Impactor using solid agar and a BioSampler liquid impactor. We performed the most probable number of assays to detect DCTB, using media supplemented with <jats:italic>Mycobacterium tuberculosis<\/jats:italic> culture filtrate (CF).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Briefly, 63% of patients (<jats:italic>n<\/jats:italic>=17) had advanced TB, and 55.6% (<jats:italic>n<\/jats:italic>=15) had a 3+ sputum smear for acid-fast bacilli. Evidence for DCTB was found in 8\u2009patients\u2019 aerosols (29.5%) and more than half of the 19 sputum samples tested (<jats:italic>n<\/jats:italic>=10; 52.6%). Two patients had DCTB in only one of the collected samples (cough aerosols or sputum). Among cough aerosol specimens, two patients (7%) only had CF-dependent DCTB.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> We detected DCTB in sputum and evidence for their presence in cough samples from pulmonary TB patients. These data suggest that bacilli undetected by traditional mycobacterial cultures may be aerosolized from pulmonary TB patients.<\/jats:p>","DOI":"10.1099\/jmm.0.002027","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T15:15:32Z","timestamp":1749222932000},"source":"Crossref","is-referenced-by-count":2,"title":["Differentially culturable Mycobacterium tuberculosis in cough-generated aerosols of patients with pulmonary tuberculosis DCTB in cough-generated aerosols"],"prefix":"10.1099","volume":"74","author":[{"given":"Luiz Guilherme Schmidt","family":"Castellani","sequence":"first","affiliation":[{"name":"N\u00facleo de Doen\u00e7as Infecciosas, Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal do Esp\u00edrito Santo, Vit\u00f3ria, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela Negrelli","family":"Brunetti","sequence":"additional","affiliation":[{"name":"N\u00facleo de Doen\u00e7as Infecciosas, Centro de 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