{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T21:03:10Z","timestamp":1773262990550,"version":"3.50.1"},"reference-count":24,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French National Reference Center for bacterial STIs"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,3]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Sexually transmitted infections (STIs) are a worldwide health issue with a high number of asymptomatic cases and the possibility of multiple infections.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Gap statement.<\/jats:bold> New multiplex real-time PCR kits targeting pathogens involved in nonviral STIs are regularly launched, but only some of them have been evaluated in comparative studies.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> This study evaluated the clinical performance of two multiplex real-time PCR commercial kits for the detection of <jats:italic>Chlamydia trachomatis<\/jats:italic>, <jats:italic>Neisseria gonorrhoeae<\/jats:italic>, <jats:italic>Mycoplasma genitalium<\/jats:italic> and <jats:italic>Trichomonas vaginalis<\/jats:italic>: the Bosphore STD Urethritis Mini Bundle Kit (BK; Anatolia Geneworks) and the Viasure Sexually Transmitted Disease Real-Time PCR Detection Kit (VK; CerTest BIOTEC).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> A total of 240 clinical specimens were evaluated. The results were compared with those of the Cobas CT\/NG and TV\/MG kits (Roche Diagnostics), used as reference methods.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Positive agreement ranged between 83.3% and 87.8% for the detection of <jats:italic>C. trachomatis<\/jats:italic>, <jats:italic>N. gonorrhoeae<\/jats:italic> and <jats:italic>T. vaginalis<\/jats:italic> using validated specimen types. For <jats:italic>M. genitalium<\/jats:italic> detection, positive agreement was 83.0% for the BK and 68.1% for the VK, which missed 31.9% of <jats:italic>M. genitalium<\/jats:italic>-positive specimens. Negative agreement ranged between 98.4% and 100% across the targeted micro-organisms. Both kits were easy to use and compatible with several DNA extraction and PCR thermal cyclers. The VK also detected the genital commensal bacteria <jats:italic>Ureaplasma<\/jats:italic> spp. and <jats:italic>Mycoplasma hominis<\/jats:italic>, which should not be targeted in STI detection kits.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Both kits are convenient methods and showed good performance for the detection of nonviral STIs, but users should be aware of a lower sensitivity of the VK for the detection of <jats:italic>M. genitalium<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.002037","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T08:16:52Z","timestamp":1751530612000},"source":"Crossref","is-referenced-by-count":2,"title":["Clinical evaluation of two commercial PCR kits for the detection of nonviral sexually transmitted infections"],"prefix":"10.1099","volume":"74","author":[{"given":"Alexandre","family":"Gaudin","sequence":"first","affiliation":[{"name":"Bacteriology Department, National Reference Centre for Bacterial Sexually Transmitted Infections, Bordeaux University Hospital, Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nad\u00e8ge","family":"H\u00e9nin","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234 Fundamental Microbiology and Pathogenicity, University of Bordeaux, Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Gardette","sequence":"additional","affiliation":[{"name":"Bacteriology Department, National Reference Centre for Bacterial Sexually Transmitted Infections, Bordeaux University Hospital, Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6065-2332","authenticated-orcid":false,"given":"C\u00e9cile","family":"B\u00e9b\u00e9ar","sequence":"additional","affiliation":[{"name":"CNRS UMR 5234 Fundamental Microbiology and Pathogenicity, University of Bordeaux, Bordeaux, 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