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A major factor in HAIs is the colonization of indwelling medical devices by biofilm-forming opportunistic pathogens such as <jats:italic>Candida albicans<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic>. These organisms frequently co-infect, resulting in synergistic interactions with enhanced virulence and resistance to treatment.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Hypothesis\/Gap statement.<\/jats:bold>\n                  <jats:italic>C. albicans<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> readily form dual-species biofilms on silicone elastomers, a commonly used medical device material, yet the colonization phenotypes of these organisms on such surfaces remain poorly understood.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> We aimed to develop a simple, optically tractable model to mimic the colonization of indwelling medical devices to investigate <jats:italic>C. albicans<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> biofilm formation.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methodology.<\/jats:bold> The system utilizes discs of a silicone elastomer embedded in agar, reflecting device-associated conditions and enabling high-resolution imaging of biofilms formed by <jats:italic>C. albicans<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> co-cultures.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Initial results using the silicone elastomer colonization model reveal robust biofilm formation. These biofilms exhibited morphological differences between dual-species biofilms formed by <jats:italic>S. aureus<\/jats:italic> co-cultures with either yeast- or hyphal-form <jats:italic>C. albicans,<\/jats:italic> indicating the impact of differing <jats:italic>C. albicans<\/jats:italic> cell morphotypes in biofilm-associated medical device colonization on silicone elastomers. Quantification of biofilm formation by crystal violet staining provided further validation of the system.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> These findings underscore the importance of developing tools for biofilm study which more closely resemble the infectious microenvironment, with our work detailing such a system which can be employed in further study to improve strategies against device-related HAIs.<\/jats:p>","DOI":"10.1099\/jmm.0.002047","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T10:16:18Z","timestamp":1752488178000},"source":"Crossref","is-referenced-by-count":6,"title":["A simple silicone elastomer colonization model highlights complexities of Candida albicans and Staphylococcus aureus interactions in biofilm 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