{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T23:55:50Z","timestamp":1793404550106,"version":"4.5.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003793","name":"Hj\u00e4rt-Lungfonden","doi-asserted-by":"crossref","award":["F2022\/2227"],"award-info":[{"award-number":["F2022\/2227"]}],"id":[{"id":"10.13039\/501100003793","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005390","name":"Alfred \u00d6sterlunds Stiftelse","doi-asserted-by":"crossref","award":["F2022\/190"],"award-info":[{"award-number":["F2022\/190"]}],"id":[{"id":"10.13039\/501100005390","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100009235","name":"Stiftelsen Riksf\u00f6rbundet Cystisk Fibros Forskningsfond","doi-asserted-by":"crossref","award":["F2022\/625"],"award-info":[{"award-number":["F2022\/625"]}],"id":[{"id":"10.13039\/501100009235","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"crossref","award":["81248"],"award-info":[{"award-number":["81248"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Swedish government funds for clinical research","award":["ALF, grant 40204"],"award-info":[{"award-number":["ALF, grant 40204"]}]},{"name":"Knut and Alice Wallenberg Foundation, medical faculty at Lund University and Region Sk\u00e5ne","award":["81234"],"award-info":[{"award-number":["81234"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:p>\n                  <jats:bold>Background.<\/jats:bold>\n                  <jats:italic>Achromobacter xylosoxidans<\/jats:italic> and <jats:italic>Pseudomonas aeruginosa<\/jats:italic> are two pathogens that cause persistent airway infections in individuals with cystic fibrosis (CF). The persistence of <jats:italic>P. aeruginosa<\/jats:italic> is partly due to a high capacity to form biofilms and the ability to exert antagonism against other bacteria. Loss of microbial diversity in conjunction with chronic <jats:italic>P. aeruginosa<\/jats:italic> colonization is strongly correlated with low lung function in CF. <jats:italic>A. xylosoxidans<\/jats:italic> and <jats:italic>P. aeruginosa<\/jats:italic> are frequently co-isolated in CF airway cultures. This study aims to investigate the reciprocal effects on growth inhibition and biofilm formation between <jats:italic>P. aeruginosa<\/jats:italic> and <jats:italic>A. xylosoxidans in vitro<\/jats:italic>.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Method.<\/jats:bold> Six isolates of <jats:italic>A. xylosoxidans<\/jats:italic>, isolated from three CF patients in early and late stages of a chronic infection, were cultured together with a CF isolate of <jats:italic>P. aeruginosa<\/jats:italic>. Biofilm formation was assessed using a microtiter assay and crystal violet staining. Quantitative PCR was used to quantify species proportions in biofilms. Growth curves were performed to compare planktonic growth rates.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Three <jats:italic>A. xylosoxidans<\/jats:italic> isolates, all of which were from early-stage infections, inhibited biofilm formation of <jats:italic>P. aeruginosa<\/jats:italic>. The inhibition was concentration-dependent and required the interaction of live bacteria during the early stages of biofilm development. The inhibitory effect was not caused by nutrient depletion of the planktonic cells. The selected <jats:italic>A. xylosoxidans<\/jats:italic> isolate had a stronger capacity to adhere to plastic surfaces compared to the <jats:italic>P. aeruginosa<\/jats:italic> isolate.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions<\/jats:bold>\n                  <jats:italic>\n                     <jats:bold>.<\/jats:bold> A. xylosoxidans<\/jats:italic> can inhibit <jats:italic>P. aeruginosa<\/jats:italic> biofilm formation <jats:italic>in vitro<\/jats:italic>. The observed effect requires active interactions between live cells during the attachment stage of biofilm formation, possibly due to differences in adhesion capacity.<\/jats:p>","DOI":"10.1099\/jmm.0.002051","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T17:15:16Z","timestamp":1754068516000},"source":"Crossref","is-referenced-by-count":4,"title":["The cystic fibrosis pathogen Achromobacter xylosoxidans inhibits biofilm formation of Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"74","author":[{"given":"Cecilia","family":"Sahl","sequence":"first","affiliation":[{"name":"Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden"},{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Andersson","sequence":"additional","affiliation":[{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Larsson","sequence":"additional","affiliation":[{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Paulsson","sequence":"additional","affiliation":[{"name":"Clinical Microbiology laboratory, Sk\u00e5ne University Hospital, Lund, Sweden"},{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oonagh","family":"Shannon","sequence":"additional","affiliation":[{"name":"Section for Oral Biology and Pathology, Faculty of Odontology, Malm\u00f6 University, Malm\u00f6, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6366-2309","authenticated-orcid":false,"given":"Lisa I.","family":"P\u00e5hlman","sequence":"additional","affiliation":[{"name":"Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden"},{"name":"Division of Infectious Diseases, Sk\u00e5ne University Hospital Lund, Lund, Sweden"},{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00810-3"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00432-15"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3390\/genes11050536"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.03.003"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2007.12.001"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2014.11.017"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.824042"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20155097"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2006.05.011"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201701-071OC"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00523-21"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136790"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2009.10.005"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00195-23"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1182-5"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2022.11.001"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.558160"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000406"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.558160"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.01.008"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01086-2017"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2023.01.001"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00037-23"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-111"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14510"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-018-0585-4"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01358-22"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.002051?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T17:15:17Z","timestamp":1754068517000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.002051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,1]]},"references-count":27,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.002051","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,1]]}}}