{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T08:53:57Z","timestamp":1787993637564,"version":"build-2784847793"},"reference-count":10,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,30]]},"abstract":"<jats:p>\n                    <jats:italic>Mycobacterium avium<\/jats:italic>\n                    complex disease is difficult to treat, with high failure and recurrence rates despite multidrug, macrolide-based treatments. The bacterial mechanisms involved in this drug tolerance and persistence are incompletely understood. Recent evidence has suggested persistence through metabolic adaptations indicative of the viable but nonculturable state, including a decreased respiratory rate and a switch to lipid accumulation and metabolism. To assess the contribution of switching to viable but nonculturable state to macrolide tolerance, we performed time\u2013kill kinetics assays for clarithromycin against\n                    <jats:italic>M. avium<\/jats:italic>\n                    . In these experiments, we performed Auramine-O (for acid-fastness, representing active mycobacteria) and Nile red (for lipid accumulation) staining and stimulation using resuscitation-promoting factors of\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    . Loss of auramine staining, increased Nile red staining and increased population sizes after stimulation with resuscitation-promoting factors support the hypothesis that clarithromycin induces a viable but nonculturable state in\n                    <jats:italic>M. avium<\/jats:italic>\n                    . Induction of a viable but nonculturable state is one of the mechanisms of macrolide tolerance in\n                    <jats:italic>M. avium<\/jats:italic>\n                    . It might be one of the drivers of the high failure and recurrence rates of macrolide-based treatments. Antimicrobials active against viable but nonculturable\n                    <jats:italic>M. avium<\/jats:italic>\n                    may improve treatment outcomes.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.002072","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:16:20Z","timestamp":1761844580000},"source":"Crossref","is-referenced-by-count":2,"title":["A viable but nonculturable state of Mycobacterium avium in response to macrolide antibiotics: a recipe for relapses?"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9306-4717","authenticated-orcid":false,"given":"Iris","family":"Schuiermanni","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Terschl\u00fcsen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrieke","family":"de Man","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Gelderse Vallei Hospital, Willy Brandtlaan 10, 6710 HN, Ede, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7250-5647","authenticated-orcid":false,"given":"Jelmer","family":"Raaijmakers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0195-5434","authenticated-orcid":false,"given":"Sandra","family":"Salillas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Paediatrics, Radiology and Public Health, Faculty of Medicine, University of Zaragoza, Zaragoza 50009, Spain"},{"name":"Department of Medical Microbiology, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7236-8934","authenticated-orcid":false,"given":"Jodie A.","family":"Schildkraut","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"},{"name":"Department of Medical Microbiology, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0581-2003","authenticated-orcid":false,"given":"Jakko","family":"van Ingen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Community for Infectious Diseases, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00535-2020"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2018.01.024"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2023.07.007"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-5-1581"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02730-20"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006077"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01509-21"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2021.08.046"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.tbtb2-0003-2015"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012595"}],"container-title":["Journal of Medical 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