{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:44:54Z","timestamp":1775123094223,"version":"3.50.1"},"reference-count":16,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pathology Queensland","award":["Research Fellowship"],"award-info":[{"award-number":["Research Fellowship"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,22]]},"abstract":"<jats:p>\n                  <jats:bold>Background.<\/jats:bold> Melioidosis is a potentially life-threatening infectious disease. The diagnosis of melioidosis is time-critical due to the organism\u2019s intrinsic antimicrobial resistance and requirement for directed therapy.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Aim.<\/jats:bold> To assess the ability of an automated molecular diagnostic instrument to detect <jats:italic>Burkholderia pseudomallei<\/jats:italic> directly from clinical samples.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods.<\/jats:bold> Urine, sputum, swabs and Ashdown\u2019s (ASH) broth were spiked with known concentrations of <jats:italic>B. pseudomallei<\/jats:italic> and analysed using an automated PCR platform (Panther<jats:sup>\u00ae<\/jats:sup> Fusion; Hologic) targeting the type III Secretion System (TTS-1) gene. In addition, clinical specimens from patients with confirmed melioidosis were also evaluated.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results.<\/jats:bold> Urine was the clinical sample that demonstrated the lowest limit of detection (LOD), 1.8\u00d710<jats:sup>2<\/jats:sup>\u2009c.f.u.\u2009ml<jats:sup>\u22121<\/jats:sup>. Compared with dry swabs (LOD: 1.0\u00d710<jats:sup>3<\/jats:sup>\u2009c.f.u.\u2009ml<jats:sup>\u22121<\/jats:sup>), Amies agar swabs were inferior (LOD: &gt;3.3\u00d710<jats:sup>4<\/jats:sup>\u2009c.f.u.\u2009ml<jats:sup>\u22121<\/jats:sup>). Inoculation of dry swabs into ASH, with an abbreviated incubation period, did not improve detection. All culture-positive sputum and urine samples from patients with confirmed melioidosis were detected by the PCR method.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> This study demonstrates the ability of the Panther<jats:sup>\u00ae<\/jats:sup> to directly detect <jats:italic>B. pseudomallei<\/jats:italic> across a range of clinical sample types and estimates the minimum bacterial concentration required for diagnostic detection. The described methodology holds promise for expediting diagnosis and, in turn, enhancing patient outcomes.<\/jats:p>","DOI":"10.1099\/jmm.0.002074","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T17:16:09Z","timestamp":1758561369000},"source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of an automated molecular diagnostic instrument for direct detection of Burkholderia pseudomallei from clinical specimens"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3548-6963","authenticated-orcid":false,"given":"Ian","family":"Gassiep","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Mater Hospital Brisbane, Brisbane, Queensland, Australia"},{"name":"Pathology Queensland, Royal Brisbane & Women\u2019s Hospital, Queensland, Brisbane, Australia"},{"name":"Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Royal Brisbane & Women\u2019s Hospital, Queensland, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Glover","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Townsville University Hospital, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Beecham","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Cairns Hospital, Cairns, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Gorman","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Royal Brisbane & Women\u2019s Hospital, Queensland, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Page","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Royal Brisbane & Women\u2019s Hospital, Queensland, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Cairns Hospital, Cairns, Queensland, Australia"},{"name":"Pathology Queensland, Cairns Hospital, Cairns, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2895-0345","authenticated-orcid":false,"given":"Patrick N.A.","family":"Harris","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Royal Brisbane & Women\u2019s Hospital, Queensland, Brisbane, Australia"},{"name":"Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Royal Brisbane & Women\u2019s Hospital, Queensland, Brisbane, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-19"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.21-0548"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed8110481"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00378-6"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02002-17"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2020-0202"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.85-90.2006"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000333"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2013.04.007"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00022-0"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0633"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2024.03.035"},{"key":"R13","first-page":"781","article-title":"Lack 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