{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T10:33:16Z","timestamp":1758191596668,"version":"3.44.0"},"reference-count":24,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["RL5GM118981"],"award-info":[{"award-number":["RL5GM118981"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["TL4GM118983"],"award-info":[{"award-number":["TL4GM118983"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["1UL1GM118982"],"award-info":[{"award-number":["1UL1GM118982"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Society of Infectious Diseases Pharmacists","award":["YIRA2025"],"award-info":[{"award-number":["YIRA2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,17]]},"abstract":"<jats:p>Contemporary pharmaceutical design often incorporates functional groups to improve the pharmacokinetic\/pharmacodynamic profile of a desired active chemical entity. These compounds are known as prodrugs. While prodrug enhancements may improve a medication\u2019s clinical utility, they often limit the ability for <jats:italic>in vitro<\/jats:italic> testing of the active drug. Published protocols suggest that commercially available phosphatase enzymes can provide a straightforward and cost-effective way to access the active components of phosphoramidate prodrugs. Here, we demonstrate that commercial phosphatases lack reproducibility in generating the active antibiotic ceftaroline from its prodrug ceftaroline fosamil (Teflaro<jats:sup>\u00ae<\/jats:sup>). We propose that previously reported successes with phosphatase-mediated conversion are due to the purification of natural ceftaroline fosamil degradation products or batch-dependent isozyme contaminants present in alkaline phosphatases obtained from biological sources. Here, we demonstrate the chemical\/thermodegradation to provide a robust, non-enzymatic source of the ceftaroline free base. This efficient method can be readily adapted to expand the availability of deprotected thermostable commercial pharmaceutical compounds for <jats:italic>in vitro<\/jats:italic> testing and research purposes.<\/jats:p>","DOI":"10.1099\/jmm.0.002077","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:16:48Z","timestamp":1758125808000},"source":"Crossref","is-referenced-by-count":0,"title":["Rapid deprotection and purification of ceftaroline free base from its phosphoramidate prodrug"],"prefix":"10.1099","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7360-9640","authenticated-orcid":false,"given":"Souvik","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5018-6497","authenticated-orcid":false,"given":"Robert D.","family":"Kina","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3665-9210","authenticated-orcid":false,"given":"Nathaniel N.","family":"Koloian","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5054-8501","authenticated-orcid":false,"given":"Daniah","family":"Zubair","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8904-5789","authenticated-orcid":false,"given":"Alexis A.","family":"Brecht","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4702-4504","authenticated-orcid":false,"given":"Andrew M.","family":"Lipchik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5658-1973","authenticated-orcid":false,"given":"Andrew D.","family":"Berti","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, School of Medicine, Wayne State University, Detroit, MI 48201, USA"},{"name":"Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-024-00914-7"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2021.1922385"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3390\/ph17060718"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202004694"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202401781"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.2174\/0929867329666220909122820"},{"key":"R7","article-title":"The phosphoramidase competency of prototypical phosphatase catalytic motifs","author":"Haney","year":"2013"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw201"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01270-19"},{"key":"R10","article-title":"New Drug Application: Teflaro (ceftaroline fosamil, NDA 200327)","author":"Cox","year":"2010"},{"key":"R11","article-title":"Etest\u00ae Ceftaroline (0.002 \u2013 32 \u00b5g\/mL) (510(k) k121002)","author":"Hojvat","year":"2012"},{"key":"R12","article-title":"bioM\u00e9rieux Releases Etest\u00ae Ceftaroline","year":"2013"},{"key":"R13","article-title":"Class 3 Device Recall Etest Ceftaroline (CPT32) US B100 (Z-0233-2015)","year":"2014"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.08.022"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-3981-8_13"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00088-14"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.56.1406"},{"key":"R18","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. 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