{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:36:09Z","timestamp":1790454969329,"version":"4.1.0"},"reference-count":28,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"\n Introduction.<\/jats:bold>\n Gastrointestinal infections remain a leading cause of morbidity and mortality within the UK.\n <\/jats:p>\n \n Hypothesis.<\/jats:bold>\n The Luminex\n \u00ae<\/jats:sup>\n NxTAG\n \u00ae<\/jats:sup>\n Gastrointestinal Pathogen Panel (NxTAG GPP) multiplex reverse transcriptase PCR assay performs equivalently to standard-of-care diagnostic approaches.\n <\/jats:p>\n \n Aim.<\/jats:bold>\n To compare the analytical performance of the NxTAG GPP assay versus routine diagnostic testing methods in a district general hospital setting.\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n Gastrointestinal pathogens in 159 faecal specimens from hospital inpatients and outpatient clinics were comparatively analysed using the NxTAG GPP assay versus traditional culture, enzyme immunoassay and molecular methods.\n <\/jats:p>\n \n Results.<\/jats:bold>\n Positive results were detected in 45 out of 159 specimens (28.3%) by NxTAG GPP, which was a higher positivity rate when compared with traditional diagnostic methods which detected 31 out of 159 (19.5%) positive infections (\n P<\/jats:italic>\n =0.087 by Fisher\u2019s exact test). Infections were caused by a single organism in 40 out of 45 (88.9%) cases, but 5 out of 45 (11.1%) infections detected were due to coinfections. No coinfections were detected by traditional methods.\n Campylobacter<\/jats:italic>\n Group was the most common enteropathogen detected with 15 out of 52 (28.9%) infections. Viruses caused 26.9% of infections, including 15.4% being norovirus. Overall sensitivity, specificity and accuracy for the NxTAG GPP assay were 97.6%, 99.7% and 99.5%, respectively, for enteropathogenic bacteria and viruses detected during this study. No parasites were detected during this study and were not included in comparisons.\n <\/jats:p>\n \n Conclusions.<\/jats:bold>\n The NxTAG GPP assay demonstrated high sensitivity and specificity for identifying gastrointestinal pathogens, with comparable accuracy as more resource-intensive and time-consuming standard diagnostic approaches. The NxTAG GPP has the potential to enhance patient diagnosis, reduce turnaround time and improve clinical outcomes compared to routine diagnostic methods.\n <\/jats:p>","DOI":"10.1099\/jmm.0.002089","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T17:17:16Z","timestamp":1762276636000},"source":"Crossref","is-referenced-by-count":1,"title":["Comparison of the Luminex\u00ae NxTAG\u00ae Gastrointestinal Pathogen Panel to traditional diagnostic methods for detecting diarrhoea-associated gastroenteritis"],"prefix":"10.1099","volume":"74","author":[{"given":"Kym","family":"Wilson","sequence":"first","affiliation":[{"name":"Microbiology Department, Derbyshire Pathology, Chesterfield Royal Hospital, Calow Chesterfield, Derbyshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Beckett","sequence":"additional","affiliation":[{"name":"Microbiology Department, Derbyshire Pathology, Chesterfield Royal Hospital, Calow Chesterfield, Derbyshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Collins","sequence":"additional","affiliation":[{"name":"Microbiology Department, Derbyshire Pathology, Chesterfield Royal Hospital, Calow Chesterfield, Derbyshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.18203\/2394-6040.ijcmph20184250"},{"key":"R2","article-title":"The third study of infectious intestinal disease in the UK (IID3)","year":"2021"},{"key":"R3","article-title":"Clostridioides difficile infection","author":"Mada","year":"2024"},{"key":"R4","doi-asserted-by":"crossref","DOI":"10.46756\/sci.fsa.squ824","article-title":"Foodborne disease estimates for the United Kingdom in 2018","author":"Holland","year":"2020"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofv113"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.18502\/ijm.v12i4.3932"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8753284"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31823096a8"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200006293422601"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis299"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8135724"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1039\/c6cs00693k"},{"key":"R13","article-title":"BD MAXTM enteric bacterial panel. 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