{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T17:15:58Z","timestamp":1776446158737,"version":"3.51.2"},"reference-count":24,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013494","name":"British Orthopaedic Foot and Ankle Society","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100013494","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,17]]},"abstract":"<jats:p>\n                    Diabetic foot disease is a major public health problem, with an annual National Health Service (NHS) expenditure exceeding \u00a31\u2009billion. Infection increases the risk of major amputation fivefold. Due to the polymicrobial nature of diabetic foot infections, it is often difficult to correctly and rapidly isolate pathological organisms with conventional culture techniques and deliver appropriate narrow-spectrum antimicrobials. Rapid DNA-based technology, using multi-channel arrays, offers a quicker alternative and has previously been used effectively in other settings. We undertook a prospective cohort study of deep tissue samples taken from diabetic foot ulcers (DFUs), comparing samples processed by conventional culture and real-time PCR TaqMan array card (TAC). Fifty samples were taken from 39\u2009patients. The ulcers were of variable chronicity prior to sampling (range: 1\u2013113\u2009weeks) and were sited on the heel (3), midfoot (6) and forefoot (41). TAC results were available an average of 4.3\u2009days earlier than culture results. Seventeen samples had the same organisms detected on culture and TAC. Sixteen of these 17 had additional organisms detected by TAC. The most frequent organisms detected by TAC that were not detected by culture were staphylococci,\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    spp.,\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    spp. and fungi. TAC rapidly and accurately detects clinically relevant organisms from DFUs, providing earlier results than standard culture. This may enable earlier rationalization of antimicrobials and infection control interventions.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.002113","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T16:17:48Z","timestamp":1776442668000},"source":"Crossref","is-referenced-by-count":0,"title":["Use of TaqMan array card to investigate bacterial isolates in diabetic foot ulcers"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4807-7416","authenticated-orcid":false,"given":"Sarah","family":"Johnson-Lynn","sequence":"first","affiliation":[{"name":"Department of Trauma and Orthopaedics, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK"},{"name":"University of York, The James Cook University Hospital, Middlesbrough, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin D.","family":"Curran","sequence":"additional","affiliation":[{"name":"Clinical Microbiology & Public Health Laboratory, United Kingdom Health Security Agency (UKHSA), Cambridge University Hospitals NHS, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Candice","family":"Allen","sequence":"additional","affiliation":[{"name":"Wolfson Diabetes and Endocrine Clinic, Institute of Metabolic Science, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine M.","family":"Webber","sequence":"additional","affiliation":[{"name":"Clinical Microbiology & Public Health Laboratory, United Kingdom Health Security Agency (UKHSA), Cambridge University Hospitals NHS, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mailis","family":"Maes","sequence":"additional","affiliation":[{"name":"Clinical Microbiology & Public Health Laboratory, United Kingdom Health Security Agency (UKHSA), Cambridge University Hospitals NHS, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Routledge","sequence":"additional","affiliation":[{"name":"Clinical Microbiology & Public Health Laboratory, United Kingdom Health Security Agency (UKHSA), Cambridge University Hospitals NHS, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Enoch","sequence":"additional","affiliation":[{"name":"Clinical Microbiology & Public Health Laboratory, United Kingdom Health Security Agency (UKHSA), Cambridge University Hospitals NHS, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew H. N.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Trauma and Orthopaedics, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony P.","family":"Coll","sequence":"additional","affiliation":[{"name":"Wolfson Diabetes and Endocrine Clinic, Institute of Metabolic Science, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","article-title":"National diabetes foot care audit - 2014-2016","year":"2017"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2022.108222"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3389\/fsurg.2021.655902"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201904_17471"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(24)00110-4"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1186\/s12902-022-00957-0"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3201\/eid2805.211153"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001218"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.17099.3"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2004.01275.x"},{"key":"R11","article-title":"Investigation of tissues and biopsies from deep-seated sites and organs (UK SMI B17)","year":"2025"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12121701"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-023-04303-1"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mim.2015.06.002"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v12.i9.1539"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2011.00720.x"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2012.0397"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.2834"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2544-1"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-016-0250-7"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.18231\/2394-5478"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2015.24.5.189"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2428-4"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.2337\/dc06-0116"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.002113?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T16:17:49Z","timestamp":1776442669000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.002113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,17]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4,17]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.002113","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,17]]}}}