{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T01:05:51Z","timestamp":1785805551021,"version":"3.56.0"},"reference-count":24,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,13]]},"abstract":"\n Introduction.<\/jats:bold>\n Rectovaginal colonization with\n Streptococcus agalactiae<\/jats:italic>\n in pregnant women is a risk factor for invasive infections of the newborns. Various culture-based approaches are available (selective vs. non-selective media\u00b1enrichment).\n <\/jats:p>\n \n Hypothesis\/Gap Statement.<\/jats:bold>\n Comprehensive head-to-head comparisons of culture-based methods for\n S. agalactiae<\/jats:italic>\n screening in pregnant women are largely missing.\n <\/jats:p>\n \n Aim.<\/jats:bold>\n We compared the test accuracy of six culture-based approaches for the detection of\n S. agalactiae<\/jats:italic>\n .\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n We performed a cross-sectional study on vaginal or rectovaginal swabs (in liquid Amies medium) from pregnant women. Samples were analysed in parallel in six study arms using (i) colistin nalidixic acid (CNA) agar, (ii) chromogenic\n S. agalactiae<\/jats:italic>\n selective agar, (iii) CNA agar after enrichment in thioglycolate broth, (iv) chromogenic agar after enrichment in thioglycolate broth, (v) CNA agar after enrichment in Todd\u2013Hewitt broth and (vi) chromogenic agar after enrichment in Todd\u2013Hewitt broth. The test accuracy was calculated for each study arm using a composite reference standard.\n <\/jats:p>\n \n Results.<\/jats:bold>\n In total, 244 pregnant women were included (median age: 32\u2009years, median weeks of pregnancy: 28 3\/7). Positivity was comparable between approaches with direct inoculation on solid media (study arms i and ii, 15.6\u201318.0%) and with primary enrichment (study arms iii\u2013vi, 17.6\u201318.9%). Each study arm had a specificity of 100%, while the sensitivity varied between 81% (CNA agar alone, study arm i) and 98% (Todd\u2013Hewitt broth for enrichment followed by subculture on chromogenic agar, study arm vi).\n <\/jats:p>\n \n Conclusion.<\/jats:bold>\n Enrichment in Todd\u2013Hewitt broth followed by subculture on chromogenic agar had the highest sensitivity (98%, specificity: 100%) for the detection of\n S. agalactiae<\/jats:italic>\n in pregnant women.\n <\/jats:p>","DOI":"10.1099\/jmm.0.002115","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T17:17:11Z","timestamp":1768324631000},"source":"Crossref","is-referenced-by-count":2,"title":["Streptococcus agalactiae colonization in pregnant women: which culture-based detection method is best?"],"prefix":"10.1099","volume":"75","author":[{"given":"Sarah","family":"Schoeler","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Theiler","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareike","family":"M\u00f6llers","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana Diana","family":"Olaru","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Schuler","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster, M\u00fcnster, 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