{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:54:42Z","timestamp":1781542482477,"version":"3.54.5"},"reference-count":26,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,15]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction.<\/jats:bold>\n                    Matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for rapid micro-organism identification and has recently been explored for antifungal susceptibility testing (AFST).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Hypothesis.<\/jats:bold>\n                    Although MALDI-TOF MS has emerged as a promising tool for AFST, simplified and clinically applicable strategies for rapid fluconazole (FLZ) susceptibility detection in\n                    <jats:italic>Candida<\/jats:italic>\n                    spp. remain insufficiently validated. We hypothesized that a streamlined AFST-MS approach would demonstrate good categorical agreement (CA) with the European Committee on Antimicrobial Susceptibility Testing (EUCAST) reference method while significantly reducing turnaround time.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    To establish a simplified MALDI-TOF MS-based AFST approach for detecting FLZ resistance in\n                    <jats:italic>Candida<\/jats:italic>\n                    species.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methodology.<\/jats:bold>\n                    Fifty-one clinical isolates and reference strains were incubated for 3 h in the presence of FLZ at two concentrations (32 and 4 \u00b5g ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and in drug-free controls. Spectral profiles were compared with the EUCAST reference method.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    Overall CA between AFST-MS and EUCAST was 85.2% (\u03ba=0.7306). Species-specific accuracy was 100% for\n                    <jats:italic>Candida auris, Pichia kudriavzevii<\/jats:italic>\n                    (formerly\n                    <jats:italic>Candida krusei<\/jats:italic>\n                    ),\n                    <jats:italic>Candida tropicalis<\/jats:italic>\n                    and\n                    <jats:italic>Candida parapsilosis<\/jats:italic>\n                    ; 92.9% for\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and 40% for\n                    <jats:italic>Nakaseomyces glabrata<\/jats:italic>\n                    (formerly\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    ); however, these estimates should be interpreted cautiously given the limited number of isolates per species. All discrepancies were minor errors, with no major or very major errors observed. The method reduced analysis time from 24 to 3 h and enabled presumptive FLZ susceptibility detection with good overall agreement with the reference method\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    These findings support the potential of MALDI-TOF MS as a rapid adjunct tool for antifungal susceptibility assessment and may contribute to earlier therapeutic decision-making.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.002172","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:15:52Z","timestamp":1781540152000},"source":"Crossref","is-referenced-by-count":0,"title":["A simplified MALDI-TOF MS method for rapid fluconazole susceptibility testing in Candida species"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4869-6660","authenticated-orcid":false,"given":"B\u00e1rbara Cipulo","family":"Legab\u00e3o","sequence":"first","affiliation":[{"name":"Pathology Department \u2013 School of Medical Sciences - University of Campinas, School of Medical Sciences, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7848-298X","authenticated-orcid":false,"given":"Jo\u00e3o Pedro Guedes","family":"Xavier","sequence":"additional","affiliation":[{"name":"Pathology Department \u2013 School of Medical Sciences - University of Campinas, School of Medical Sciences, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9584-434X","authenticated-orcid":false,"given":"Ana Luisa Perini Leme","family":"Giordano","sequence":"additional","affiliation":[{"name":"Pathology Department \u2013 School of Medical Sciences - University of Campinas, School of Medical Sciences, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6172-3219","authenticated-orcid":false,"given":"Lais","family":"Pontes","sequence":"additional","affiliation":[{"name":"Pathology Department \u2013 School of Medical Sciences - University of Campinas, School of Medical Sciences, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7737-3575","authenticated-orcid":false,"given":"Mauro Luiz","family":"Brand\u00e3o Junior","sequence":"additional","affiliation":[{"name":"Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5095-5054","authenticated-orcid":false,"given":"Ang\u00e9lica Zaninelli","family":"Schreiber","sequence":"additional","affiliation":[{"name":"Pathology Department \u2013 School of Medical Sciences - University of Campinas, School of Medical Sciences, Campinas, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-023-00926-2"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13020272"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-024-00503-3"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1531543"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2022.102237"},{"key":"R6","article-title":"Definitive document E.Def 7.4. 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