{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T14:49:44Z","timestamp":1782139784627,"version":"3.54.5"},"reference-count":39,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["T32AI055449"],"award-info":[{"award-number":["T32AI055449"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI193182"],"award-info":[{"award-number":["R21AI193182"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI176089"],"award-info":[{"award-number":["R21AI176089"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,22]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction<\/jats:bold>\n                    . Multidrug-resistant infections by the opportunistic pathogen\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    are common in healthcare settings but are increasingly difficult to treat due to high rates of antimicrobial resistance.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Hypothesis\/Gap Statement<\/jats:bold>\n                    . Interest has developed in using R pyocins, bacteriocins produced by\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , as a species-specific treatment. We hypothesize that understanding the connections between disease type and R pyocin sensitivity will inform future treatment development.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim<\/jats:bold>\n                    . To evaluate the patterns of R pyocin sensitivity across a library of 247 clinical\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    isolates and compare sensitivity profiles of strains when categorizing based on disease type, O-antigen serotype and encoded R pyocin type.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methodology<\/jats:bold>\n                    . A combination of genomic analysis and R pyocin sensitivity assays using growth kinetics was conducted on 247 clinical isolates to evaluate genotype, phenotype and disease presentation in the context of R pyocins. Genomic analysis allowed\n                    <jats:italic>in silico<\/jats:italic>\n                    identification of O-antigen serotype and encoded R pyocin type, and phylogenetic comparisons revealed genetic relationships between strains.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    . Comparative analysis of clinical isolates showed that certain conditions, such as chronic infection in the lungs of patients with cystic fibrosis, increased sensitivity to R pyocins. We also observed strong correlations between encoded R pyocin subtype, sensitivity and O-antigen serotype. Finally, our data showed that a large percentage of strains had shared, broad-spectrum sensitivity to R pyocins, suggesting that most clinical isolates may be treatable with R pyocins.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    . Our study reveals connections between disease type, O-antigen serotype and R pyocin sensitivity, which may inform future strain analysis and predict the effectiveness of R pyocin treatment.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.002179","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T14:16:28Z","timestamp":1782137788000},"source":"Crossref","is-referenced-by-count":0,"title":["R pyocin sensitivity of Pseudomonas aeruginosa clinical isolates from different disease types"],"prefix":"10.1099","volume":"75","author":[{"given":"Yanhan","family":"Deng","sequence":"first","affiliation":[{"name":"Department of BioSciences, Rice University, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meggie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of BioSciences, Rice University, Houston, TX, 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