{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:46Z","timestamp":1792786186082,"version":"4.5.0"},"reference-count":35,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Trinity College Dublin","award":["Provost's PhD Project Award 2020\/21"],"award-info":[{"award-number":["Provost's PhD Project Award 2020\/21"]}],"id":[{"id":"https:\/\/ror.org\/02tyrky19","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,24]]},"abstract":"<jats:p>\n                    Infections caused by multidrug-resistant\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    are considered a threat to human and animal health. The widely studied\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    strain AB5075 displays a high degree of antibiotic resistance. In this study, we experimentally validated that antibiotic resistance is largely mediated by resistance genes located on plasmid p1AB5075. We used a p1AB5075-deficient AB5075 strain to assess individual contributions of p1AB5075-encoded antibiotic resistance genes by ectopically (over-)expressing each gene in the \u0394p1AB5075 background. By determining individual contributions of seven p1AB5075-encoded antibiotic resistance genes, we show individual and overlapping roles of genes for aminoglycoside resistance and uncover the importance of extended-spectrum\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -lactamase\n                    <jats:italic>\n                      bla\n                      <jats:sub>GES-11<\/jats:sub>\n                    <\/jats:italic>\n                    for monobactam and cephalosporin resistance in\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AB5075. We discovered that aminoglycoside\n                    <jats:italic>N<\/jats:italic>\n                    -acetyltransferase\n                    <jats:italic>aaC(6\u2032)-Ib3<\/jats:italic>\n                    (\n                    <jats:italic>aacA4<\/jats:italic>\n                    ), which was previously shown to confer resistance to tobramycin, provides broad resistance to gentamicin, kanamycin, amikacin, streptomycin and tobramycin when overexpressed in\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AB5075. Because p1AB5075 is transferable to a wide range of environmental and clinical\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    strains and non-\n                    <jats:italic>baumannii Acinetobacter<\/jats:italic>\n                    species, the relevance of our findings extends beyond\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    AB5075.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.002189","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T09:16:21Z","timestamp":1784884581000},"source":"Crossref","is-referenced-by-count":1,"title":["Contributions of plasmid p1AB5075-encoded antibiotic resistance genes to multidrug resistance of Acinetobacter baumannii AB5075"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1443-9877","authenticated-orcid":false,"given":"Orlaith","family":"Plunkett","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Genetics & Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1014-7677","authenticated-orcid":false,"given":"Anna S.","family":"Ershova","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Genetics & Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1453-9002","authenticated-orcid":false,"given":"Kristina","family":"Schauer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Genetics & 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