{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:23:39Z","timestamp":1787844219607,"version":"build-2784847793"},"reference-count":50,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,27]]},"abstract":"\n Introduction.<\/jats:bold>\n Klebsiella<\/jats:italic>\n spp. are the second most common uropathogens after\n Escherichia coli<\/jats:italic>\n and are globally recognized for their high morbidity, driven by their significant ability to develop multi-drug resistance against a wide range of antimicrobials.\n <\/jats:p>\n \n Hypothesis\/Gap Statement.<\/jats:bold>\n In Central Africa, and particularly in Gabon, there is a significant lack of data regarding the prevalence and genetic diversity of uropathogenic\n Klebsiella<\/jats:italic>\n spp. resistant to last-line antibiotics involved in urinary tract infections (UTIs) in women.\n <\/jats:p>\n \n Aim.<\/jats:bold>\n This study aims to characterize the resistance of\n Klebsiella<\/jats:italic>\n spp. involved in UTIs in women to last-line therapeutic molecules and to explore their genetic diversity through enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) profiling.\n <\/jats:p>\n \n Methodology.<\/jats:bold>\n The germs were isolated on Urilines biphasic slides. Identification and susceptibility profiles were performed using the API 20E, ATB Ur and VITEK\n \u00ae<\/jats:sup>\n 2 COMPACT. Molecular research of carbapenemase genes (\n bla<\/jats:italic>\n KPC<\/jats:sub>\n ,\n bla<\/jats:italic>\n IMP<\/jats:sub>\n and\n bla<\/jats:italic>\n \n VIM<\/jats:italic>\n <\/jats:sub>\n ) and resistance to fosfomycin (\n fos<\/jats:italic>\n A,\n fos<\/jats:italic>\n B,\n fos<\/jats:italic>\n C,\n glpT<\/jats:italic>\n and\n uhpT<\/jats:italic>\n ) was made by conventional PCR, and genetic diversity was screened using ERIC-PCR.\n <\/jats:p>\n \n Results.<\/jats:bold>\n Of 343\u2009patients, 60.64% (208 out of 343) were women, with a prevalence of UTIs of 43.75% (91 out of 208). The involvement of\n Klebsiella<\/jats:italic>\n spp. uropathogens was 29.03% (27 out of 93) against 53.76% (50 out of 93) of other enterobacteria.\n Klebsiella<\/jats:italic>\n spp. strains showed high levels of resistance to third-generation cephalosporins (55.56%), fourth-generation cephalosporins and fosfomycin (40.74%), average levels of resistance to third-generation fluoroquinolones (25.92%) and low levels of resistance to carbapenems (7.41%). A total of 81.82% (18 out of 22) of\n Klebsiella<\/jats:italic>\n spp. strains possess antibiotic resistance genes,\n bla<\/jats:italic>\n KPC<\/jats:sub>\n (40.0%) and\n fos<\/jats:italic>\n A (36.0%), respectively. The study of genetic diversity by interpretation of ERIC-PCR profiles shows 19 distinct banding patterns and 2 repeated profiles, all divided into 9 clusters.\n <\/jats:p>\n \n Conclusion.<\/jats:bold>\n Uropathogenic\n Klebsiella<\/jats:italic>\n spp. isolated from women have high rates of resistance to last-line antibiotics, with the exception of imipenem. They exhibit diverse ERIC-PCR profiles and possess\n bla<\/jats:italic>\n KPC<\/jats:sub>\n and\n fos<\/jats:italic>\n A. These findings provide important preliminary surveillance data for the region.\n <\/jats:p>","DOI":"10.1099\/jmm.0.002190","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T15:15:30Z","timestamp":1787843730000},"source":"Crossref","is-referenced-by-count":0,"title":["Characterization of resistance to last-line therapeutic molecules and genetic diversity of Klebsiella spp. strains involved in urinary tract infections in women: the case of H\u00f4pital d\u2019Instruction des Arm\u00e9es Omar Bongo Ondimba"],"prefix":"10.1099","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0970-0208","authenticated-orcid":false,"given":"Evrard","family":"Mayombo Ngoussou","sequence":"first","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie Unit\u00e9 de Recherche d\u2019Analyses M\u00e9dicales, Centre Interdisciplinaire de Recherches M\u00e9dicales de Franceville, BP: 769, Franceville, 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