{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:44:33Z","timestamp":1792514673801,"version":"4.5.0"},"reference-count":44,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hologic Inc","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction.<\/jats:bold>\n                    Molecular assays such as the Hologic Aptima\n                    <jats:sup>\u00ae<\/jats:sup>\n                    Vaginitis Panel and Seegene Allplex\n                    <jats:sup>\u2122<\/jats:sup>\n                    Vaginitis assay offer improved sensitivity and multiplex detection for the diagnosis of vaginitis compared with the non-molecular standard of care.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Gap Statement.<\/jats:bold>\n                    Evidence comparing the relative performance of Aptima versus Seegene is limited, as is knowledge of Aptima\u2019s compatibility with non-proprietary swabs.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aim.<\/jats:bold>\n                    This prospective study assessed the diagnostic performance of the Aptima BV (bacterial vaginosis) and Aptima CV\/TV assays (\n                    <jats:italic>Candida<\/jats:italic>\n                    vaginitis\/\n                    <jats:italic>Trichomonas<\/jats:italic>\n                    vaginitis) alongside the Seegene Allplex assay in symptomatic women (\u226518\u2009years) presenting for vaginitis testing in primary care in France.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methodology.<\/jats:bold>\n                    Two vaginal swabs were taken per patient: one Aptima Multitest Swab and one non-proprietary \u03a3-TRANSWAB liquid Amies collection swab. Aptima swab data were used solely to confirm performance of the Aptima assays under current test conditions, while all primary assay comparisons were performed using \u03a3-TRANSWAB samples. These comparisons were performed for each assay relative to Nugent score (for BV) or culture (for CV), as well as Aptima relative to Seegene.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results.<\/jats:bold>\n                    In total, 500\u2009women underwent vaginitis testing between July and August 2024. When using non-proprietary \u03a3-TRANSWAB collection methods, sensitivity and specificity values for the Aptima BV assay were 96.7 and 89.7%\u2009relative to Nugent score, and 89.9 and 90.6% for Seegene Allplex relative to Nugent score. Among \u2018intermediate BV\u2019 samples (\n                    <jats:italic>n<\/jats:italic>\n                    =66), agreement between Seegene Allplex and Nugent score was 1.5%. Sensitivity and specificity values for the detection of CV were 88.6 and 90.8% for the Aptima CV\/TV assay, and 90.2 and 93.7% for Seegene Allplex. Assay detection of TV could not be validated due to an insufficient number of TV-positive samples (\n                    <jats:italic>n<\/jats:italic>\n                    =1). These findings were consistent with Aptima\u2019s performance when using proprietary collection methods.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    Overall, the Aptima Vaginitis Panel demonstrated comparably high diagnostic performance to Seegene Allplex for the detection of BV and CV when using a non-proprietary sample collection method. TV prevalence was too low in this cohort to permit meaningful assay evaluation for this pathogen.\n                  <\/jats:p>","DOI":"10.1099\/jmm.0.002210","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:17:12Z","timestamp":1792513032000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Hologic Aptima\u00ae Vaginitis and Seegene Allplex\u2122 assays for microbiological diagnosis of bacterial vaginosis and Candida vaginitis in women in France, relative to Nugent score and culture"],"prefix":"10.1099","volume":"75","author":[{"given":"C\u00e9cile","family":"Martaresche","sequence":"first","affiliation":[{"name":"Clinical Laboratory, INOVIE Barla, Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Scherrer","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, INOVIE Barla, Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","volume-title":"Vaginitis","author":"Hildebrand","year":"2025"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2021.09.022"},{"key":"R3","first-page":"321","article-title":"Vaginitis: diagnosis and treatment","volume":"97","author":"Paladine","year":"2018","journal-title":"Am Fam Physician"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00075-15"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1097\/01.JAA.0000526770.60197.fa"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.631972"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3390\/jof6010027"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001178"},{"key":"R9","volume-title":"Vaginal Candidiasis","author":"Jeanmonod","year":"2024"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000618"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7184.192121"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2022.10.009"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1177\/09564624221103035"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11102470"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.2.297-301.1991"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1136\/sti.78.6.413"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1177\/0956462418785451"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/365194"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00342-18"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.672429"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.3390\/jof1020217"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01643-19"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050893"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03480-8"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01264-19"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03694-w"},{"key":"R27","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1111\/1471-0528.16661","article-title":"Clinical validation of a new molecular test (Seegene AllplexTM Vaginitis) for the diagnosis of vaginitis: a cross-sectional study","volume":"128","author":"Vieira-Baptista","year":"2021","journal-title":"Bjog"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01748-22"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00837-22"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25010449"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12010111"},{"key":"R32","article-title":"Aptima Vaginal Health"},{"key":"R33","article-title":"Panther Scalable Solutions"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01301-22"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.115953"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01274-24"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.002163"},{"key":"R38","article-title":"AllplexTM Vaginitis Screening Assay"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11020191"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2025.106610"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.759697"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200104000-00002"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e318167b105"},{"key":"R44","article-title":"Article L1121-1 - 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