{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T07:12:45Z","timestamp":1781853165966,"version":"3.54.5"},"reference-count":33,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,6,1]]},"abstract":"<jats:p><jats:italic>Paracoccidioides brasiliensis<\/jats:italic>yeast cells can enter mammalian cells and may manipulate the host cell environment to favour their own growth and survival. Moreover, fibronectin and several other host extracellular matrix proteins are recognized by various components of the yeast cell extracts. The present study was designed to isolate and characterize a fibronectin-binding protein from<jats:italic>P. brasiliensis<\/jats:italic>. We also compared<jats:italic>P. brasiliensis<\/jats:italic>strain 18, tested before (Pb18a) and after (Pb18b) animal passage, in relation to its adhesion and invasion processes. Extracts from both samples, when cultured on blood agar solid medium, showed higher levels of protein expression than when the same samples were cultured on Fava-Netto solid medium, as demonstrated by two-dimensional electrophoresis and SDS-PAGE. Also, both Pb18a and Pb18b exhibited stronger adhesion to A549 epithelial cells when cultured on blood agar medium than when cultured on Fava-Netto medium. Ligand affinity binding assays revealed a protein of 54\u2005kDa and pI 5.6 in<jats:italic>P. brasiliensis<\/jats:italic>cell-free extracts with the properties of a fibronectin-binding adhesin, which was characterized by tryptic digestion and mass spectroscopy as a homologue of enolase from<jats:italic>P. brasiliensis<\/jats:italic>. Antibody raised against this 54\u2005kDa protein abolished 80\u200a% of<jats:italic>P. brasiliensis<\/jats:italic>adhesion to A549 epithelial cells. Our results demonstrate that<jats:italic>P. brasiliensis<\/jats:italic>produces a fibronectin-binding adhesin, irrespective of the culture medium, and that this activity can be inhibited by a specific antibody and is involved in the adhesion of the fungus to pulmonary epithelial cells.<\/jats:p>","DOI":"10.1099\/jmm.0.003830-0","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T17:23:51Z","timestamp":1241803431000},"page":"706-713","source":"Crossref","is-referenced-by-count":62,"title":["Enolase from Paracoccidioides brasiliensis: isolation and identification as a fibronectin-binding protein"],"prefix":"10.1099","volume":"58","author":[{"given":"Fabiana Cristina","family":"Donofrio","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, UNESP, Araraquara, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina Alvarez","family":"Calil","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, UNESP, Araraquara, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine Toscano","family":"Miranda","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, UNESP, Araraquara, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Marisa Fusco","family":"Almeida","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Faculdade de Ci\u00eancias Farmac\u00eauticas, UNESP, Araraquara, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gil","family":"Benard","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Dermatologia e Imunodefici\u00eancias, Faculdade de Medicina, 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