{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T00:53:07Z","timestamp":1717980787864},"reference-count":55,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,2,1]]},"abstract":"<jats:p>The significance of<jats:italic>Neisseria meningitidis<\/jats:italic>serogroup B membrane proteins as vaccine candidates is continually growing. Here, we studied different aspects of antigen NMB0088, a protein that is abundant in outer-membrane vesicle preparations and is thought to be a surface protein. The gene encoding protein NMB0088 was sequenced in a panel of 34 different meningococcal strains with clinical and epidemiological relevance. After this analysis, four variants of NMB0088 were identified; the variability was confined to three specific segments, designated VR1, VR2 and VR3. Secondary structure predictions, refined with alignment analysis and homology modelling using FadL of<jats:italic>Escherichia coli<\/jats:italic>, revealed that almost all the variable regions were located in extracellular loop domains. In addition, the NMB0088 antigen was expressed in<jats:italic>E. coli<\/jats:italic>and a procedure for obtaining purified recombinant NMB0088 is described. The humoral immune response elicited in BALB\/c mice was measured by ELISA and Western blotting, while the functional activity of these antibodies was determined in a serum bactericidal assay and an animal protection model. After immunization in mice, the recombinant protein was capable of inducing a protective response when it was administered inserted into liposomes. According to our results, the recombinant NMB0088 protein may represent a novel antigen for a vaccine against meningococcal disease. However, results from the variability study should be considered for designing a cross-protective formulation in future studies.<\/jats:p>","DOI":"10.1099\/jmm.0.004820-0","type":"journal-article","created":{"date-parts":[[2009,1,13]],"date-time":"2009-01-13T21:08:39Z","timestamp":1231880919000},"page":"196-208","source":"Crossref","is-referenced-by-count":17,"title":["Neisseria meningitidis antigen NMB0088: sequence variability, protein topology and vaccine potential"],"prefix":"10.1099","volume":"58","author":[{"given":"Gretel","family":"Sardi\u00f1as","sequence":"first","affiliation":[{"name":"Meningococcal Research Department, Division of Vaccines, Center for Genetic Engineering and Biotechnology, Avenue 31, Cubanacan, Habana 10600, Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Yero","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Division of Biotechnology, Finlay Institute, Avenue 27, La Lisa, Habana 11600, 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