{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:03:10Z","timestamp":1761393790483},"reference-count":17,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,5,1]]},"abstract":"<jats:p>We developed a new quantification method for the measles virus (MeV) nucleoprotein (N) gene using real-time reverse transcriptase PCR. This method allowed us to quantify 10<jats:sup>1<\/jats:sup>\u201310<jats:sup>7<\/jats:sup>\u2005copies per reaction (corresponding to 5\u00d710<jats:sup>\u22121<\/jats:sup>\u20135\u00d710<jats:sup>5<\/jats:sup>\u2005copies\u200a\u03bcl<jats:sup>\u22121<\/jats:sup>) of the MeV N gene. We also quantified the MeV N gene from the throat swabs of 22 patients with measles as well as the MeV genotypes A, D3, D5, D9 and H1 in viral suspensions derived from MeV-infected cells. As a result, 3.9\u00d710<jats:sup>3<\/jats:sup>\u20135.2\u00d710<jats:sup>6<\/jats:sup>\u2005copies\u2005ml<jats:sup>\u22121<\/jats:sup>and 7.4\u00d710<jats:sup>7<\/jats:sup>\u20132.0\u00d710<jats:sup>8<\/jats:sup>\u2005copies\u2005ml<jats:sup>\u22121<\/jats:sup>of the MeV genomes (N gene) were detected in the throat swabs and viral suspensions, respectively. No other viruses (enteroviruses, respiratory syncytial virus, human metapneumovirus or mumps virus) were detected in the assay. The results suggest that this method is applicable to the detection and quantification of some genotypes of MeV.<\/jats:p>","DOI":"10.1099\/jmm.0.005439-0","type":"journal-article","created":{"date-parts":[[2009,4,15]],"date-time":"2009-04-15T17:38:13Z","timestamp":1239817093000},"page":"638-643","source":"Crossref","is-referenced-by-count":5,"title":["Development of an assay for the detection and quantification of the measles virus nucleoprotein (N) gene using real-time reverse transcriptase PCR"],"prefix":"10.1099","volume":"58","author":[{"given":"Miho","family":"Akiyama","sequence":"first","affiliation":[{"name":"Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Kimura","sequence":"additional","affiliation":[{"name":"Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, 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