{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:28:44Z","timestamp":1759991324600},"reference-count":23,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,5,1]]},"abstract":"<jats:p>One hundred and one randomly selected (2003\u20132005) clinical isolates of<jats:italic>Pseudomonas aeruginosa<\/jats:italic>were used to assess the quantitative (MIC) and qualitative (susceptibility category) agreement between the microdilution broth reference method (RM) and disc diffusion (DD), Etest and the VITEK 2 automated susceptibility test system for determination of the susceptibility of<jats:italic>P. aeruginosa<\/jats:italic>to piperacillin (PIP), PIP\u2013tazobactam (TZP), ceftazidime (CAZ), aztreonam (ATM) cefepime (FEP) and imipenem (IMP). The results obtained by the RM were compared with those obtained by the other methods. The RM and DD were performed according to CLSI criteria. Etest and VITEK 2 were according to the manufacturer's instructions. The Advanced Expert System (AES), which interprets MICs generated by VITEK 2, was modified with new rules of interpretation. Overall, VITEK 2 showed the lowest MIC<jats:sub>90<\/jats:sub>values for the six antibiotics. The RM categorical testing (susceptibility and resistance) rates with<jats:italic>P. aeruginosa<\/jats:italic>were 11.8 and 88.1 for PIP, 22.7 and 77.2 for TZP, 14.8 and 78.2 for CAZ, 12.8 and 54.4 for ATM, 16.8 and 75.3 for FEP, and 7.9 and 90.1 for IMP, respectively. Very major errors (false susceptible) were only detected for ATM and FEP with DD and for IMP with three methods. Major errors (false resistant) were generally acceptable for all antibiotics except TZP. VITEK 2 yielded a high level of minor errors (trends toward false susceptibility), mainly with CAZ and FEP. A good agreement was obtained for all antibiotics\/methods assayed, thus highlighting the importance of the AES for categorization of<jats:italic>\u03b2<\/jats:italic>-lactam susceptibility in<jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.005587-0","type":"journal-article","created":{"date-parts":[[2009,4,15]],"date-time":"2009-04-15T17:38:13Z","timestamp":1239817093000},"page":"625-629","source":"Crossref","is-referenced-by-count":13,"title":["Comparison of different methods of determining \u03b2-lactam susceptibility in clinical strains of Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"58","author":[{"given":"Eva","family":"Torres","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda \u2013 Unidad de Investigaci\u00f3n, Complejo Hospitalario Universitario Juan Canalejo, La Coru\u00f1a, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Villanueva","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda \u2013 Unidad de Investigaci\u00f3n, Complejo Hospitalario Universitario Juan Canalejo, La Coru\u00f1a, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germ\u00e1n","family":"Bou","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda \u2013 Unidad de Investigaci\u00f3n, Complejo Hospitalario Universitario Juan Canalejo, La Coru\u00f1a, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0732-8893(94)00069-9","article-title":"Interpretive errors using an automated system for the susceptibility testing of imipenem and aztreonam","volume":"21","author":"Biedenbach","year":"1995","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R2","first-page":"305","article-title":"Accuracy of cefepime antimicrobial susceptibility testing results for Pseudomonas aeruginosa tested on the MicroScan WalkAway system","volume":"33","author":"Biedenbach","year":"1999","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R3","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0732-8893(01)00234-6","article-title":"Comparison of two commercial systems (Vitek and MicroScan-WalkAway) for antimicrobial susceptibility testing of Pseudomonas aeruginosa isolates from cystic fibrosis patients","volume":"39","author":"Burns","year":"2001","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R4","article-title":"Performance Standards for Antimicrobial Susceptibility Testing . 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