{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:28:55Z","timestamp":1787941735081,"version":"build-2784847793"},"reference-count":17,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,6,1]]},"abstract":"<jats:p>Phenotypic identification of AmpC, KPC and extended-spectrum <jats:italic>\u03b2<\/jats:italic>-lactamases (ESBLs) among members of the <jats:italic>Enterobacteriaceae<\/jats:italic> remains challenging. This study compared the Phoenix Automated Microbiology System (BD Diagnostics) with the Clinical and Laboratory Standards Institute confirmatory method to identify ESBL production among 200 <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Klebsiella pneumoniae<\/jats:italic> clinical isolates. The Phoenix system misclassified nearly half of the isolates as ESBL-positive, requiring manual testing for confirmation. Inclusion of aztreonam\u00b1clavulanic acid (CA) and cefpodoxime\u00b1CA in the testing algorithm increased the ESBL detection rate by 6\u200a%. Boronic acid-based screening identified 24 isolates as AmpC<jats:sup>+<\/jats:sup>, but in a subset of genotypically characterized isolates, appeared to have a high false-positivity rate. PCR screening revealed eight KPC<jats:sup>+<\/jats:sup> isolates, all of which tested as ESBL<jats:sup>+<\/jats:sup> or ESBL<jats:sup>+<\/jats:sup> AmpC<jats:sup>+<\/jats:sup> by phenotypic methods, but half were reported as carbapenem-susceptible by the Phoenix system. Overall, these results indicate that laboratories should use the Phoenix ESBL results only as an initial screen followed by confirmation with an alternative method.<\/jats:p>","DOI":"10.1099\/jmm.0.006171-0","type":"journal-article","created":{"date-parts":[[2009,5,8]],"date-time":"2009-05-08T17:23:51Z","timestamp":1241803431000},"page":"774-778","source":"Crossref","is-referenced-by-count":21,"title":["Performance of the Phoenix bacterial identification system compared with disc diffusion methods for identifying extended-spectrum \u03b2-lactamase, AmpC and KPC producers"],"prefix":"10.1099","volume":"58","author":[{"given":"Mark A.","family":"Fisher","sequence":"first","affiliation":[{"name":"ARUP Laboratories, Salt Lake City, UT, USA"},{"name":"University of Utah, School of Medicine, Salt Lake City, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Stamper","sequence":"additional","affiliation":[{"name":"The Johns Hopkins Hospital, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine M.","family":"Hujer","sequence":"additional","affiliation":[{"name":"Louis Stokes VA Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Love","sequence":"additional","affiliation":[{"name":"Case Western Reserve University, School of Medicine, Cleveland, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Croft","sequence":"additional","affiliation":[{"name":"ARUP Laboratories, Salt Lake City, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Cohen","sequence":"additional","affiliation":[{"name":"ARUP Laboratories, Salt Lake City, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Case Western Reserve University, School of Medicine, Cleveland, OH, USA"},{"name":"Louis Stokes VA Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[{"name":"The Johns Hopkins Hospital, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy A.","family":"Petti","sequence":"additional","affiliation":[{"name":"ARUP Laboratories, Salt Lake City, UT, USA"},{"name":"University of Utah, School of Medicine, Salt Lake City, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1128\/JCM.00015-07","article-title":"Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae","volume":"45","author":"Anderson","year":"2007","journal-title":"J Clin Microbiol"},{"key":"R2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing ; 18th Informational Supplement. 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