{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T16:16:31Z","timestamp":1732032991730},"reference-count":46,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"abstract":"The serotyping of O and H antigens is an important first step in the characterization of Salmonella enterica<\/jats:italic>. However, serotyping has become increasingly technically demanding and expensive to perform. We have therefore sequenced additional S. enterica<\/jats:italic> O antigen gene clusters to provide information for the development of DNA-based serotyping methods. Three S. enterica<\/jats:italic> isolates had O antigen gene clusters with homology to the Escherichia coli<\/jats:italic> O123 O antigen region. O antigen clusters from two serogroup O58 S. enterica<\/jats:italic> strains had approximately 85\u200a% identity with the E. coli<\/jats:italic> O123 O antigen region over their entire length, suggesting that these Salmonella<\/jats:italic> and E. coli<\/jats:italic> O antigen regions evolved from a common ancestor. The O antigen cluster of a Salmonella<\/jats:italic> serogroup O41 isolate had a lower level of identity with E. coli<\/jats:italic> O123 over only part of its O antigen DNA cluster sequence, suggesting a different and more complex evolution of this gene cluster than those in the O58 strains. A large part of the Salmonella<\/jats:italic> O41 O antigen DNA cluster had very close identity with the O antigen cluster of an O62 strain. This region of DNA homology included the wzx<\/jats:italic> and wzy<\/jats:italic> genes. Therefore, molecular serotyping tests using only the O41 or O62 wzx<\/jats:italic> and wzy<\/jats:italic> genes would not differentiate between the two serogroups. The E. coli<\/jats:italic> O123 O-antigenic polysaccharide and its repeating unit were characterized, and the chemical structure for E. coli<\/jats:italic> O123 was entirely consistent with the O antigen gene cluster sequences of E. coli<\/jats:italic> O123 and the Salmonella<\/jats:italic> O58 isolates. An understanding of both the genetic and structural composition of Salmonella<\/jats:italic> and E. coli<\/jats:italic> O antigens is necessary for the development of novel molecular methods for serotyping these organisms.<\/jats:p>","DOI":"10.1099\/jmm.0.007187-0","type":"journal-article","created":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T17:47:00Z","timestamp":1244742420000},"page":"884-894","source":"Crossref","is-referenced-by-count":7,"title":["Escherichia coli O123 O antigen genes and polysaccharide structure are conserved in some Salmonella enterica serogroups"],"prefix":"10.1099","volume":"58","author":[{"given":"Clifford G.","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, 510 Medical Sciences Building, University of Manitoba, 730 William Avenue, Winnipeg, MB R3T 2N2, Canada"},{"name":"Enteric Diseases Program, Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew M.","family":"Kropinski","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, ON N1G 3W4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haralambos","family":"Parolis","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Rhodes University, Grahamstown 6140, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher C. 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