{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:47:31Z","timestamp":1768481251038,"version":"3.49.0"},"reference-count":20,"publisher":"Microbiology Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"abstract":"<jats:p>Clinical isolates of<jats:italic>Mycobacterium avium<\/jats:italic>(<jats:italic>n<\/jats:italic>=81) from patients with pulmonary infections who were HIV-negative and isolates (<jats:italic>n<\/jats:italic>=33) from HIV-positive patients were subjected to genetic analysis by PCR detection of three<jats:italic>M. avium<\/jats:italic>-specific insertion sequences (IS<jats:italic>901<\/jats:italic>, IS<jats:italic>1245<\/jats:italic>and IS<jats:italic>1311<\/jats:italic>), and nucleotide sequencing of the heat-shock protein 65 (<jats:italic>hsp65<\/jats:italic>) gene. All clinical isolates were identified as \u2018<jats:italic>M. avium<\/jats:italic>subspecies<jats:italic>hominissuis<\/jats:italic>\u2019 by sequence analysis of<jats:italic>hsp65<\/jats:italic>. Compared with clinical isolates of<jats:italic>M. avium<\/jats:italic>reported elsewhere, IS<jats:italic>1245<\/jats:italic>was found less frequently in Japanese isolates (96\/114 isolates, 84\u200a%) and IS<jats:italic>901<\/jats:italic>was detected more frequently (76\/114 isolates, 67\u200a%). One isolate was found to lack IS<jats:italic>1311<\/jats:italic>, which has not been reported previously for \u2018<jats:italic>M. avium<\/jats:italic>subsp.<jats:italic>hominissuis<\/jats:italic>\u2019. Nucleotide sequence analysis of the PCR products for IS<jats:italic>901<\/jats:italic>revealed that all clinical isolates had the same new insertion sequence, designated IS<jats:italic>Mav6<\/jats:italic>, which had 60 point mutations compared with the nucleotide sequence of the original IS<jats:italic>901<\/jats:italic>. These results suggest that \u2018<jats:italic>M. avium<\/jats:italic>subsp.<jats:italic>hominissuis<\/jats:italic>\u2019 with IS<jats:italic>Mav6<\/jats:italic>is prevalent in Japan. IS<jats:italic>Mav6<\/jats:italic>may have implications for the virulence of<jats:italic>M. avium<\/jats:italic>and contribute to an increase of<jats:italic>M. avium<\/jats:italic>infections in this country.<\/jats:p>","DOI":"10.1099\/jmm.0.008623-0","type":"journal-article","created":{"date-parts":[[2009,6,11]],"date-time":"2009-06-11T17:47:00Z","timestamp":1244742420000},"page":"945-950","source":"Crossref","is-referenced-by-count":34,"title":["Characterization of Mycobacterium avium clinical isolates in Japan using subspecies-specific insertion sequences, and identification of a new insertion sequence, ISMav6"],"prefix":"10.1099","volume":"58","author":[{"given":"Kazuya","family":"Ichikawa","sequence":"first","affiliation":[{"name":"Department of Clinical Research, National Hospital Organization, Higashinagoya National Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya, Aichi 468-8620, Japan"},{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Aichi 468-8503, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Yagi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Center of National University Hospital for Infection Control, Nagoya University Hospital, 65 Tsurumai, Showa-ku, Nagoya, Aichi 466-8560, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Moriyama","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, National Hospital Organization, Nagoya Medical Center, 4-1-1 Sannomaru, Naka-ku, Nagoya, Aichi 460-0001, Japan"},{"name":"Department of Clinical Research, National Hospital Organization, Higashinagoya National Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya, Aichi 468-8620, Japan"},{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Aichi 468-8503, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Inagaki","sequence":"additional","affiliation":[{"name":"Department of Clinical Research, National Hospital Organization, Higashinagoya National Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya, Aichi 468-8620, Japan"},{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Aichi 468-8503, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taku","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, National Hospital Organization, Higashinagoya National Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya, Aichi 468-8620, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kei-Ichi","family":"Uchiya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Aichi 468-8503, 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