{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:01:45Z","timestamp":1787587305497,"version":"build-2736575974"},"reference-count":31,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,11,1]]},"abstract":"The emergence of multidrug-resistant (MDR) and extended-spectrum \u03b2<\/jats:italic>-lactamase (ESBL)-producing Klebsiella pneumoniae<\/jats:italic> poses a serious antibiotic management problem as resistance genes are easily transferred from one organism to another. Fifty-one strains of K. pneumoniae<\/jats:italic> isolated from sporadic cases in various hospitals throughout Malaysia were analysed by antimicrobial susceptibility testing, PCR detection of ESBL-encoding genes and DNA fingerprinting. Although 27 of the 51 K. pneumoniae<\/jats:italic> strains were MDR (i.e. resistant to three or more classes of antibiotics), the majority of the strains (98\u200a%) were sensitive to imipenem. PCR detection using ESBL gene-specific primers showed that 46 of the K. pneumoniae<\/jats:italic> strains harboured bla<\/jats:italic>\n SHV<\/jats:sub>, 19 harboured bla<\/jats:italic>\n CTX-M<\/jats:sub>, 5 harboured bla<\/jats:italic>\n OXA-1<\/jats:sub> and 4 harboured bla<\/jats:italic>\n TEM-1<\/jats:sub>. Class 1 integron-encoded intI1<\/jats:italic> integrase was detected in 21 of the 51 K. pneumoniae<\/jats:italic> strains and amplification of the integron 5\u2032CS region showed the presence of several known antibiotic resistance gene cassettes of various sizes. Results of conjugation and transformation experiments indicated that some of the ESBL-encoding genes (i.e. bla<\/jats:italic>\n SHV<\/jats:sub>, bla<\/jats:italic>\n CTX-M<\/jats:sub> and bla<\/jats:italic>\n TEM-1<\/jats:sub>) were transmissible and were likely plasmid-encoded. DNA fingerprinting using PFGE and PCR-based methods indicated that the 51 K. pneumoniae<\/jats:italic> strains were genetically diverse and heterogeneous.<\/jats:p>","DOI":"10.1099\/jmm.0.011114-0","type":"journal-article","created":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T18:39:15Z","timestamp":1255113555000},"page":"1463-1469","source":"Crossref","is-referenced-by-count":39,"title":["Characterization of multidrug-resistant and extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals"],"prefix":"10.1099","volume":"58","author":[{"given":"King Ting","family":"Lim","sequence":"first","affiliation":[{"name":"Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chew Chieng","family":"Yeo","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Biotechnology, Universiti Darul Iman Malaysia, 20400 Kuala Terengganu, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohani","family":"Md Yasin","sequence":"additional","affiliation":[{"name":"Specialized Diagnostic Centre, Institute of Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganeswrie","family":"Balan","sequence":"additional","affiliation":[{"name":"Hospital Sultanah Aminah Johor Bharu, 80100 Johor Bharu, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwai Lin","family":"Thong","sequence":"additional","affiliation":[{"name":"Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1099\/jmm.0.04981-0","article-title":"Molecular epidemiology of an outbreak of multiresistant Klebsiella pneumoniae in a Tunisan 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