{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:48:27Z","timestamp":1761155307692},"reference-count":38,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,10,1]]},"abstract":"<jats:p>Pathogenic <jats:italic>Leptospira<\/jats:italic> is the aetiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. The search for novel antigens that could be relevant in host\u2013pathogen interactions is being pursued. These antigens have the potential to elicit several activities, including adhesion. This study focused on a hypothetical predicted lipoprotein of <jats:italic>Leptospira<\/jats:italic>, encoded by the gene LIC12895, thought to mediate attachment to extracellular matrix (ECM) components. The gene was cloned and expressed in <jats:italic>Escherichia coli<\/jats:italic> BL21 Star (DE3)pLys by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and characterized by circular dichroism spectroscopy. The capacity of the protein to mediate attachment to ECM components was evaluated by binding assays. The leptospiral protein encoded by LIC12895, named Lsa27 (leptospiral surface adhesin, 27\u2005kDa), bound strongly to laminin in a dose-dependent and saturable fashion. Moreover, Lsa27 was recognized by antibodies from serum samples of confirmed leptospirosis specimens in both the initial and the convalescent phases of the disease. Lsa27 is most likely a surface protein of <jats:italic>Leptospira<\/jats:italic> as revealed in liquid-phase immunofluorescence assays with living organisms. Taken together, these data indicate that this newly identified membrane protein is expressed during natural infection and may play a role in mediating adhesion of <jats:italic>L. interrogans<\/jats:italic> to its host.<\/jats:p>","DOI":"10.1099\/jmm.0.011916-0","type":"journal-article","created":{"date-parts":[[2009,9,11]],"date-time":"2009-09-11T16:50:48Z","timestamp":1252687848000},"page":"1275-1282","source":"Crossref","is-referenced-by-count":36,"title":["A newly identified protein of Leptospira interrogans mediates binding to laminin"],"prefix":"10.1099","volume":"58","author":[{"given":"Mariana T.","family":"Longhi","sequence":"first","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiane R.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliete C.","family":"Romero","sequence":"additional","affiliation":[{"name":"Divis\u00e3o de Biologia Medica, Instituto Adolfo Lutz, Sao Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amane P.","family":"Gon\u00e7ales","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zenaide M.","family":"de Morais","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L. 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