{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T03:50:22Z","timestamp":1793764222463,"version":"4.6.1"},"reference-count":22,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,5,1]]},"abstract":"<jats:p>A multiplex PCR is described which detects capsular types K1, K2, K5, K54 and K57, which are those most associated with invasive disease or pathogenicity, a further capsular type (K20), two putative virulence factors (<jats:italic>rmpA<\/jats:italic> and <jats:italic>wcaG<\/jats:italic>) and the 16S\u201323S internal transcribed spacer unit of <jats:italic>Klebsiella pneumoniae<\/jats:italic>, facilitating identification of this organism. <jats:italic>wcaG<\/jats:italic> encodes capsular fucose production and was associated with capsular types K1 and K54, but was also found in strains of other capsular types; 18 of the 543 isolates screened were PCR-positive for this gene. An eight-locus variable number tandem repeat (VNTR) scheme was designed, which provided discrimination at a level similar to that afforded by PFGE among a panel of 36 isolates representing 29 PFGE types. All isolates tested of the virulent K1 clone of CC23, associated with pyogenic liver abscesses, shared the same VNTR profile, which may be helpful in identifying this clone; such isolates were also PCR-positive for <jats:italic>allS<\/jats:italic>. These methods provide a rapid means of characterizing and typing isolates of this important agent of community-acquired and nosocomial infection.<\/jats:p>","DOI":"10.1099\/jmm.0.015198-0","type":"journal-article","created":{"date-parts":[[2010,1,29]],"date-time":"2010-01-29T02:52:20Z","timestamp":1264733540000},"page":"541-547","source":"Crossref","is-referenced-by-count":264,"title":["PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets"],"prefix":"10.1099","volume":"59","author":[{"given":"Jane F.","family":"Turton","sequence":"first","affiliation":[{"name":"Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London NW9 5EQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Perry","sequence":"additional","affiliation":[{"name":"Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection 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