{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T07:07:22Z","timestamp":1789628842414,"version":"build-2803163510"},"reference-count":33,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:p>The objective of this study was to determine the distribution of staphylococcal cassette chromosome <jats:italic>mec<\/jats:italic> (SCC<jats:italic>mec<\/jats:italic>) elements in meticillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from a tertiary-care hospital in Mexico and to examine the relationship to drug resistance. Fifty selected MR-CoNS isolates collected from catheters (<jats:italic>n<\/jats:italic>=15), blood (<jats:italic>n<\/jats:italic>=15), bone (<jats:italic>n<\/jats:italic>=9), bronchial lavage (<jats:italic>n<\/jats:italic>=2) and urine (<jats:italic>n<\/jats:italic>=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion, synovial fluid, tracheal aspirate and wound secretion were examined. Susceptibility testing was performed by the broth microdilution method. SCC<jats:italic>mec<\/jats:italic> types were determined by multiplex PCR and PFGE was carried out as described previously for <jats:italic>Staphylococcus aureus<\/jats:italic>. Among the MR-CoNS strains studied, the most frequently isolated species were <jats:italic>Staphylococcus epidermidis<\/jats:italic> (<jats:italic>n<\/jats:italic>=26) and <jats:italic>Staphylococcus haemolyticus<\/jats:italic> (<jats:italic>n<\/jats:italic>=13). <jats:italic>Staphylococcus cohnii<\/jats:italic> (<jats:italic>n<\/jats:italic>=5), <jats:italic>Staphylococcus hominis<\/jats:italic> (<jats:italic>n<\/jats:italic>=3), <jats:italic>Staphylococcus sciuri<\/jats:italic> (<jats:italic>n<\/jats:italic>=1), <jats:italic>Staphylococcus pasteuri<\/jats:italic> (<jats:italic>n<\/jats:italic>=1) and the recently described species <jats:italic>Staphylococcus pettenkoferi<\/jats:italic> (<jats:italic>n<\/jats:italic>=1) were also identified. The most frequent MR-CoNS genotype identified was SCC<jats:italic>mec<\/jats:italic> type IVa in <jats:italic>S. epidermidis<\/jats:italic> isolates, which also showed a high diversity in their PFGE patterns. A clone was found that amplified both SCC<jats:italic>mec<\/jats:italic> III and V elements in five isolates examined. The single MR <jats:italic>S. pettenkoferi<\/jats:italic> isolate harboured SCC<jats:italic>mec<\/jats:italic> type IVd and the single MR <jats:italic>S. pasteuri<\/jats:italic> isolate harboured SCC<jats:italic>mec<\/jats:italic> type I. The carriage of SCC<jats:italic>mec<\/jats:italic> type III was associated with resistance or intermediate resistance to meropenem (<jats:italic>P<\/jats:italic> &lt;0.05). These results confirm the high prevalence of <jats:italic>S. epidermidis<\/jats:italic> SCC<jats:italic>mec<\/jats:italic> IVa and the high genetic diversity among MR-CoNS strains. As far as is known, this is the first report describing the newly identified <jats:italic>S. pettenkoferi<\/jats:italic> possessing SCC<jats:italic>mec<\/jats:italic> IVd and <jats:italic>S. pasteuri<\/jats:italic> harbouring SCC<jats:italic>mec<\/jats:italic> type I. MR-CoNS harbouring SCC<jats:italic>mec<\/jats:italic> type III were found to be more resistant to meropenem.<\/jats:p>","DOI":"10.1099\/jmm.0.015800-0","type":"journal-article","created":{"date-parts":[[2009,12,11]],"date-time":"2009-12-11T02:29:43Z","timestamp":1260498583000},"page":"323-329","source":"Crossref","is-referenced-by-count":35,"title":["Diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance"],"prefix":"10.1099","volume":"59","author":[{"given":"Elvira","family":"Garza-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar","family":"Pezina","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Muruet","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virgilio","family":"Bocanegra-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioingenier\u00eda, UAM Reynosa Aztl\u00e1n, Universidad Aut\u00f3noma de Tamaulipas, Tamaulipas, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilo","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge M.","family":"LLaca-D\u00edaz","sequence":"additional","affiliation":[{"name":"Hospital Universitario Dr Jos\u00e9 Eleuterio Gonz\u00e1lez, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Monterrey Nuevo Leon 64460, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","volume-title":"BSAC Methods for Antimicrobial Susceptibility Testing","author":"Andrews","year":"2008"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1128\/JB.01058-08","article-title":"Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, [corrected] reflecting the ancestral genome of the human-pathogenic staphylococci","volume":"191","author":"Baba","year":"2009","journal-title":"J Bacteriol"},{"key":"R3","first-page":"390","article-title":"Staphylococcus , Micrococcus and other catalase-positive cocci","volume-title":"In Manual of Clinical Microbiology","author":"Bannerman","year":"2007"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1111\/j.1469-0691.2008.02090.x","article-title":"The origin of a methicillin-resistant Staphylococcus aureus isolate at a neonatal ward in Sweden \u2013 possible horizontal transfer of a staphylococcal cassette chromosome mec between methicillin-resistant Staphylococcus haemolyticus and Staphylococcus aureus","volume":"14","author":"Berglund","year":"2008","journal-title":"Clin Microbiol Infect"},{"key":"R5","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1128\/AAC.50.3.1001-1012.2006","article-title":"Staphylococcal cassette chromosome mec (SCC mec ) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCC mec elements","volume":"50","author":"Chongtrakool","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"R6","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; Eighth Informational Supplement , CLSI document M100-S19","year":"2009"},{"key":"R7","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1097\/QCO.0b013e3283013af4","article-title":"Recent trends and prevention of infection in the neonatal intensive care unit","volume":"21","author":"Curtis","year":"2008","journal-title":"Curr Opin Infect Dis"},{"key":"R8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.diagmicrobio.2006.08.019","article-title":"Staphylococcus haemolyticus endocarditis: clinical and microbiologic analysis of 4 cases","volume":"57","author":"Falcone","year":"2007","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R9","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1574-695X.2005.00009.x","article-title":"SCC mec in staphylococci: genes on the move","volume":"46","author":"Hanssen","year":"2006","journal-title":"FEMS Immunol Med Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1128\/JCM.01085-08","article-title":"Carriage of methicillin-resistant staphylococci and their SCC mec types in a long-term-care facility","volume":"47","author":"Ibrahem","year":"2009","journal-title":"J Clin Microbiol"},{"key":"R11","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1128\/AAC.45.5.1323-1336.2001","article-title":"Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus","volume":"45","author":"Ito","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"R12","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1128\/JCM.02262-07","article-title":"Extreme genetic diversity of methicillin-resistant Staphylococcus epidermidis strains disseminated among healthy Japanese children","volume":"46","author":"Jamaluddin","year":"2008","journal-title":"J Clin Microbiol"},{"key":"R13","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1128\/AAC.44.6.1549-1555.2000","article-title":"A new class of genetic element, staphylococcus cassette chromosome mec , encodes methicillin resistance in Staphylococcus aureus","volume":"44","author":"Katayama","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"R14","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1128\/JCM.02328-06","article-title":"First case of osteomyelitis caused by \u201c Staphylococcus pettenkoferi \u201d","volume":"45","author":"Lo\u00efez","year":"2007","journal-title":"J Clin Microbiol"},{"key":"R15","doi-asserted-by":"crossref","first-page":"e14","DOI":"10.1086\/340981","article-title":"& Hospital Infection Surveillance Team","volume":"35","author":"Lyytik\u00e4inen","year":"2002","journal-title":"Clin Infect Dis"},{"key":"R16","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1128\/AAC.46.4.1147-1152.2002","article-title":"Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains","volume":"46","author":"Ma","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"key":"R17","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1099\/jmm.0.47294-0","article-title":"Distribution of staphylococcal cassette chromosome mec (SCC mec ) types I, II, III and IV in coagulase-negative staphylococci from patients attending a tertiary hospital in southern Brazil","volume":"56","author":"Mombach Pinheiro Machado","year":"2007","journal-title":"J Med Microbiol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1128\/JCM.41.4.1574-1585.2003","article-title":"Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus , a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains","volume":"41","author":"Murchan","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R19","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1128\/JCM.30.8.2058-2063.1992","article-title":"Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination","volume":"30","author":"Musser","year":"1992","journal-title":"J Clin Microbiol"},{"key":"R20","doi-asserted-by":"crossref","first-page":"4250","DOI":"10.1073\/pnas.0306398101","article-title":"Bacterial biota in the human distal esophagus","volume":"101","author":"Pei","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R21","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1128\/AAC.00724-08","article-title":"Diversity of staphylococcal cassette chromosome mec structures in methicillin-resistant Staphylococcus epidermidis and Staphylococcus haemolyticus strains among outpatients from four countries","volume":"53","author":"Rupp\u00e9","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"R22","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.jinf.2008.10.006","article-title":"Contamination of a donated platelet unit by Staphylococcus pasteuri","volume":"57","author":"Savini","year":"2008","journal-title":"J Infect"},{"key":"R23","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1136\/jcp.2009.067041","article-title":"Staphylococcus pasteuri bacteraemia in patient with leukaemia","volume":"62","author":"Savini","year":"2009a","journal-title":"J Clin Pathol"},{"key":"R24","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1099\/jmm.0.008193-0","article-title":"Meticillin-heteroresistant Staphylococcus pasteuri from an apheresis platelet product","volume":"58","author":"Savini","year":"2009b","journal-title":"J Med Microbiol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1099\/jmm.0.004697-0","article-title":"Human bloodstream infection caused by Staphylococcus pettenkoferi","volume":"58","author":"Song","year":"2009","journal-title":"J Med Microbiol"},{"key":"R26","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1128\/JCM.33.9.2233-2239.1995","article-title":"Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing","volume":"33","author":"Tenover","year":"1995","journal-title":"J Clin Microbiol"},{"key":"R27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0732-8893(02)00399-1","article-title":"Staphylococcus pettenkoferi ,\u201d a novel staphylococcal species isolated from clinical specimens","volume":"43","author":"Tr\u00fclzsch","year":"2002","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R28","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1099\/ijs.0.64381-0","article-title":"Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens","volume":"57","author":"Tr\u00fclzsch","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"R29","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1128\/JCM.33.11.2864-2867.1995","article-title":"Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR","volume":"33","author":"Vannuffel","year":"1995","journal-title":"J Clin Microbiol"},{"key":"R30","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1179\/joc.2006.18.3.235","article-title":"Vascular catheter-associated infections: a microbiological and therapeutic update","volume":"18","author":"Viale","year":"2006","journal-title":"J Chemother"},{"key":"R31","doi-asserted-by":"crossref","first-page":"3574","DOI":"10.1128\/AAC.47.11.3574-3579.2003","article-title":"Related clones containing SCCmec type IV predominate among clinically significant Staphylococcus epidermidis isolates","volume":"47","author":"Wisplinghoff","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"R32","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.1128\/JCM.43.10.5026-5033.2005","article-title":"Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus","volume":"43","author":"Zhang","year":"2005","journal-title":"J Clin Microbiol"},{"key":"R33","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1128\/AAC.01118-08","article-title":"Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus","volume":"53","author":"Zhang","year":"2009","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.015800-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T14:22:46Z","timestamp":1601475766000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.015800-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,1]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.015800-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,1]]}}}