{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T02:03:41Z","timestamp":1792548221441,"version":"4.5.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,5,1]]},"abstract":"<jats:p>This report focuses on the molecular characterization of 22 extended-spectrum <jats:italic>\u03b2<\/jats:italic>-lactamase-producing <jats:italic>Escherichia coli<\/jats:italic> isolates collected in a German university hospital during a period of 9 months in 2006. Relationship analysis of clinical isolates was done via PFGE, multilocus sequence typing, plasmid profiling and additionally PCR for <jats:italic>bla<\/jats:italic>\n                  <jats:sub>ESBL<\/jats:sub> detection and determination of phylogroups. After conjugal transfer, plasmid isolation and subsequent PCR for <jats:italic>bla<\/jats:italic>\n                  <jats:sub>ESBL<\/jats:sub> detection and determination of incompatibility groups were performed. Using one-primer walking, up to 3600\u2005bp upstream and downstream of different <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> genes could be sequenced. <jats:italic>\u03b2<\/jats:italic>-Lactamases found were TEM-1 (<jats:italic>n<\/jats:italic>=14), SHV-5 (<jats:italic>n<\/jats:italic>=1) and a wide variety of CTX-M types (<jats:italic>n<\/jats:italic>=21), i.e. CTX-M-15 (<jats:italic>n<\/jats:italic>=12), CTX-M-1 (<jats:italic>n<\/jats:italic>=4), CTX-M-14 (<jats:italic>n<\/jats:italic>=2), CTX-M-9 (<jats:italic>n<\/jats:italic>=1), CTX-M-3 (<jats:italic>n<\/jats:italic>=1) and one new type, CTX-M-65 (<jats:italic>n<\/jats:italic>=1). In 18 isolates, <jats:italic>bla<\/jats:italic>\n                  <jats:sub>ESBL<\/jats:sub> genes were located on conjugative plasmids of sizes between 40 and 180\u2005kbp belonging to incompatibility groups FII (<jats:italic>n<\/jats:italic>=9), N (<jats:italic>n<\/jats:italic>=5) and I1 (<jats:italic>n<\/jats:italic>=4). <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> was found to be associated with the common elements IS<jats:italic>Ecp1<\/jats:italic>, IS<jats:italic>26<\/jats:italic> and IS<jats:italic>903-D<\/jats:italic>, but with unusual spacer sequences for IS<jats:italic>Ecp1<\/jats:italic> in two isolates. These insertion sequences, connected to <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> as well as other genes, were located between two IS<jats:italic>26<\/jats:italic> elements in a configuration that has not yet been described. The results reveal the emergence of <jats:italic>bla<\/jats:italic>\n                  <jats:sub>ESBL<\/jats:sub>, predominantly <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub>, located on different plasmids harboured by genotypically different <jats:italic>E. coli<\/jats:italic> strains. The identical gene arrangement in the <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> neighbourhood in plasmids of different incompatibility groups indicates a main role of IS<jats:italic>26<\/jats:italic> in distribution of mobile resistance elements between different plasmids.<\/jats:p>","DOI":"10.1099\/jmm.0.016188-0","type":"journal-article","created":{"date-parts":[[2010,1,22]],"date-time":"2010-01-22T02:09:39Z","timestamp":1264126179000},"page":"580-587","source":"Crossref","is-referenced-by-count":81,"title":["A novel IS26 structure surrounds bla\n            CTX-M genes in different plasmids from German clinical Escherichia coli isolates"],"prefix":"10.1099","volume":"59","author":[{"given":"A.","family":"Cullik","sequence":"first","affiliation":[{"name":"Robert Koch Institute, Burgstra\u00dfe 37, 38855 Wernigerode, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Burgstra\u00dfe 37, 38855 Wernigerode, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Prager","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Burgstra\u00dfe 37, 38855 Wernigerode, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"von Baum","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Steinh\u00f6velstra\u00dfe 9, 89075 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Witte","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Burgstra\u00dfe 37, 38855 Wernigerode, 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