{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T12:46:24Z","timestamp":1756385184836},"reference-count":8,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:p>Two cases involving polymicrobial culture-negative samples were investigatedby 16S rRNA gene sequencing, with analysis of mixed chromatograms. <jats:italic>Fusobacteriumnecrophorum<\/jats:italic>, <jats:italic>Prevotella intermedia<\/jats:italic> and <jats:italic>Streptococcus constellatus<\/jats:italic> were identified from pleural fluid in a patient with Lemierre'ssyndrome and <jats:italic>Neisseria meningitidis<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic>were identified from a petechia in a patient with meningococcal disease.<\/jats:p>","DOI":"10.1099\/jmm.0.017269-0","type":"journal-article","created":{"date-parts":[[2009,12,25]],"date-time":"2009-12-25T02:30:39Z","timestamp":1261708239000},"page":"486-488","source":"Crossref","is-referenced-by-count":12,"title":["Direct 16S rRNA gene sequencing of polymicrobial culture-negative sampleswith analysis of mixed chromatograms"],"prefix":"10.1099","volume":"59","author":[{"given":"Gitte N.","family":"Hartmeyer","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Odense University Hospital, Odense,Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrik S.","family":"Justesen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Odense University Hospital, 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