{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T23:08:58Z","timestamp":1773184138200,"version":"3.50.1"},"reference-count":17,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"The US Centers for Disease Control and Prevention lists Brucella<\/jats:italic> as a potential bioterrorism threat requiring enhanced diagnostic capacity and surveillance (http:\/\/emergency.cdc.gov\/bioterrorism\/<\/jats:ext-link>). Successful treatment and management of patients after exposure to biological threat agents depends on accurate and timely diagnosis, but many biothreat agents present with similar, vague clinical signs \u2013 commonly referred to as \u2018flu-like illness\u2019. Diagnosis of brucellosis is notoriously challenging, especially early in infection, and definitive diagnosis may require invasive methods, e.g. bone marrow biopsy. We studied the pathogenesis of Brucella suis<\/jats:italic> aerosol infection in rhesus macaques in an effort to guide the diagnostic algorithm in case of possible intentional exposure of humans. Rhesus proved to be an excellent model for human brucellosis; the data showed that PCR DNA amplification testing of non-invasive diagnostic samples has the potential to definitively detect a point-source outbreak immediately and for several days after exposure.<\/jats:p>","DOI":"10.1099\/jmm.0.017285-0","type":"journal-article","created":{"date-parts":[[2010,3,12]],"date-time":"2010-03-12T02:39:37Z","timestamp":1268361577000},"page":"724-730","source":"Crossref","is-referenced-by-count":20,"title":["A rhesus macaque (Macaca mulatta) model of aerosol-exposure brucellosis (Brucella suis): pathology and diagnostic implications"],"prefix":"10.1099","volume":"59","author":[{"given":"Samuel L.","family":"Yingst","sequence":"first","affiliation":[{"name":"US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis M.","family":"Huzella","sequence":"additional","affiliation":[{"name":"US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Chuvala","sequence":"additional","affiliation":[{"name":"Akimeka Technologies LLC, 1425 Porter Street, Fort Detrick, MD 21702, USA"},{"name":"US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Wolcott","sequence":"additional","affiliation":[{"name":"US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1016\/j.cgh.2006.08.010","article-title":"The liver in brucellosis","volume":"5","author":"Akritidis","year":"2007","journal-title":"Clin Gastroenterol Hepatol"},{"key":"R2","first-page":"323","article-title":"Brucellosis","volume":"12","author":"Aleixo","year":"1999","journal-title":"Acta Med Port"},{"key":"R3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1373\/clinchem.2005.052522","article-title":"Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms","volume":"52","author":"Christensen","year":"2006","journal-title":"Clin Chem"},{"key":"R4","article-title":"Guide for the Care and Use of Laboratory Animals","volume":"No. 86\u201323","year":"1996","journal-title":"National Institutes of Health Publication"},{"key":"R5","doi-asserted-by":"crossref","first-page":"224","DOI":"10.3109\/08958370903191023","article-title":"Characterization of a head-only aerosol exposure chamber for nonhuman primates","volume":"22","author":"Dabisch","year":"2010","journal-title":"Inhal Toxicol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1258\/td.2006.006266","article-title":"Brucellosis: a retrospective evaluation of 99 cases and review of brucellosis treatment","volume":"38","author":"Demirt\u00fcrk","year":"2008","journal-title":"Trop Doct"},{"key":"R7","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"Franco","year":"2007","journal-title":"Lancet Infect Dis"},{"key":"R8","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0272-2712(18)30018-0","article-title":"Clinical recognition and management of patients exposed to biological warfare agents","volume":"21","author":"Franz","year":"2001","journal-title":"Clin Lab Med"},{"key":"R9","first-page":"513","article-title":"Brucellosis. 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