{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T20:55:40Z","timestamp":1793652940604,"version":"4.5.1"},"reference-count":32,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:p>Consumption of inadequately pasteurized dairy products is the most common means of transmission of brucellosis. This report describes two foodborne outbreaks that occurred in families infected after consumption of fresh home-made cheese bought in different Argentine provinces. High resolution variable number of tandem repeats (VNTR)-based analysis revealed two well-defined groups comprising essentially identical profiles and corresponding to the two different outbreaks. Similar clinical findings in members of the same family could indicate that the differential virulence of different bacterial clones, as indicated by VNTR data, could have influenced the course of the disease. We observed the importance of adequate treatment in early stages of the disease; combination therapy and extended treatment for 6 weeks or longer yielded significantly better results. The risk of the foodborne transmission of this zoonotic disease and disease prevention should be considered.<\/jats:p>","DOI":"10.1099\/jmm.0.017525-0","type":"journal-article","created":{"date-parts":[[2010,2,19]],"date-time":"2010-02-19T02:39:30Z","timestamp":1266547170000},"page":"648-652","source":"Crossref","is-referenced-by-count":10,"title":["Application of variable number of tandem repeats typing to describe familial outbreaks of brucellosis in Argentina"],"prefix":"10.1099","volume":"59","author":[{"given":"Nidia E.","family":"Lucero","sequence":"first","affiliation":[{"name":"Brucellosis Laboratory, National Laboratories and Institutes of Health Administration (ANLIS) \u2018Dr C. G. Malbr\u00e1n\u2019, Avenida Velez Sarsfield 563, 1281 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Tenenbaum","sequence":"additional","affiliation":[{"name":"Department of Infectology, Pediatric Hospital \u2018Dr Ricardo Gutierrez\u2019, Gallo 1330, 1425 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nestor R.","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Infectology, Hospital \u2018Dr Cosme Argerich\u2019, Margall 750, 1155 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela I.","family":"Escobar","sequence":"additional","affiliation":[{"name":"Brucellosis Laboratory, National Laboratories and Institutes of Health Administration (ANLIS) \u2018Dr C. G. Malbr\u00e1n\u2019, Avenida Velez Sarsfield 563, 1281 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Groussaud","sequence":"additional","affiliation":[{"name":"Brucellosis Reference Unit, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, Addlestone, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"Brucellosis Reference Unit, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency, Woodham Lane, Addlestone, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1080\/00365520150218147","article-title":"Ascites caused by brucellosis: a report of two cases","volume":"36","author":"Akritidis","year":"2001","journal-title":"Scand J Gastroenterol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1159\/000088960","article-title":"Failure of a short-term antibiotic therapy for human brucellosis using ciprofloxacin. A study on in vitro susceptibility of Brucella strains","volume":"51","author":"Al Dahouk","year":"2005","journal-title":"Chemotherapy"},{"key":"R3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.12.015","article-title":"Evaluation of Brucella MLVA typing for humans brucellosis","volume":"69","author":"Al Dahouk","year":"2007","journal-title":"J Microbiol Methods"},{"key":"R4","first-page":"13","article-title":"Bacteriological and serological methods","volume-title":"In Techniques for the Brucellosis Laboratory","author":"Alton","year":"1988"},{"key":"R5","doi-asserted-by":"crossref","first-page":"e317","DOI":"10.1371\/journal.pmed.0040317","article-title":"Perspectives for the treatment of brucellosis in the 21st century: the Ioannina recommendations","volume":"4","author":"Ariza","year":"2007","journal-title":"PLoS Med"},{"key":"R6","first-page":"1051","article-title":"HLA-B*39 allele confers susceptibility to osteoarticular complications in human brucellosis","volume":"30","author":"Bravo","year":"2003","journal-title":"J Rheumatol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1111\/j.1399-0039.2007.00823.x","article-title":"Polymorphism of the transmembrane region of the MICA gene and human brucellosis","volume":"69","author":"Bravo","year":"2007","journal-title":"Tissue Antigens"},{"key":"R8","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cyto.2008.07.008","article-title":"TGF- \u03b2 1 and IL-6 gene polymorphism in Spanish brucellosis patients","volume":"44","author":"Bravo","year":"2008","journal-title":"Cytokine"},{"key":"R9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1471-2180-3-15","article-title":"Brucella \u201cHOOF Prints\u201d: strain typing by multilocus analysis of variable number tandem repeats (VNTRs)","volume":"3","author":"Bricker","year":"2003","journal-title":"BMC Microbiol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.cyto.2007.04.008","article-title":"IL-10 and IL-6 gene polymorphisms as potential host susceptibility factors in brucellosis","volume":"38","author":"Budak","year":"2007","journal-title":"Cytokine"},{"key":"R11","first-page":"303","article-title":"Epidemic outbreak of 81 cases of brucellosis following the consumption of fresh cheese without pasteurisation","volume":"70","author":"Castell Monsalve","year":"1996","journal-title":"Rev Esp Salud Publica"},{"key":"R12","first-page":"1","article-title":"Case definitions for infectious conditions under public health surveillance","volume":"46","year":"1997","journal-title":"MMWR Recomm Rep"},{"key":"R13","volume-title":"Brucellosis in Humans and Animals , WHO\/CDS\/EPR\/ 2006\u20137","author":"Corbel","year":"2006"},{"key":"R14","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1007\/0-387-29298-5_90","article-title":"Genus I. Brucella Meyer and Shaw 1920, 173AL","volume-title":"In Bergey\u2032s Manual of Systematic Bacteriology","volume":"vol. 2","author":"Corbel","year":"2005"},{"key":"R15","volume-title":"A Guide to the Diagnosis, Treatment, and Prevention of Human Brucellosis , VPH\/81.31","author":"Elberg","year":"1981"},{"key":"R16","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.mimet.2009.05.009","article-title":"Molecular typing for epidemiological evaluation of Brucella abortus and Brucella canis isolated in Korea","volume":"78","author":"Kang","year":"2009","journal-title":"J Microbiol Methods"},{"key":"R17","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1128\/JCM.00464-08","article-title":"Evaluation of a multilocus variable number tandem repeat analysis scheme for typing human Brucella isolates in an endemic country","volume":"46","author":"Kattar","year":"2008","journal-title":"J Clin Microbiol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1128\/JCM.36.5.1425-1427.1998","article-title":"Buffered plate antigen test as a screening test for the diagnosis of human brucellosis","volume":"36","author":"Lucero","year":"1998","journal-title":"J Clin Microbiol"},{"key":"R19","doi-asserted-by":"crossref","first-page":"3245","DOI":"10.1128\/JCM.37.10.3245-3248.1999","article-title":"Competitive enzyme immunoassay for diagnosis of human brucellosis","volume":"37","author":"Lucero","year":"1999","journal-title":"J Clin Microbiol"},{"key":"R20","doi-asserted-by":"crossref","first-page":"2923","DOI":"10.1128\/JCM.00822-07","article-title":"Molecular epidemiological and antibiotic susceptibility characterization of Brucella isolates from humans in Sicily, Italy","volume":"45","author":"Marianelli","year":"2007","journal-title":"J Clin Microbiol"},{"key":"R21","doi-asserted-by":"crossref","first-page":"164","DOI":"10.2807\/esm.08.07.00421-en","article-title":"Brucellosis outbreak due to unpasteurised raw goat cheese in Andalucia (Spain), January \u2013 March 2002","volume":"8","author":"Mendez Martinez","year":"2003","journal-title":"Euro Surveill"},{"key":"R22","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1128\/JCM.00900-09","article-title":"Molecular epidemiology of Brucella genotypes in patients at a major hospital in Central Peru","volume":"47","author":"N\u00f6ckler","year":"2009","journal-title":"J Clin Microbiol"},{"key":"R23","first-page":"1173","article-title":"International Committee on Systematics of Prokaryotes. Subcommittee on the Taxonomy of Brucella","volume":"56","author":"Osterman","year":"2006","journal-title":"Minutes of the meeting"},{"key":"R24","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1056\/NEJMra050570","article-title":"Brucellosis","volume":"352","author":"Pappas","year":"2005","journal-title":"N Engl J Med"},{"key":"R25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0378-1135(02)00247-X","article-title":"Brucellosis in Argentina","volume":"90","author":"Samartino","year":"2002","journal-title":"Vet Microbiol"},{"key":"R26","article-title":"Se autoriza la importaci\u00f3n, producci\u00f3n y el uso de la vacuna Rev. 1, en las condiciones que determinen la Direcci\u00f3n Nacional de Sanidad Animal y la Direcci\u00f3n de Laboratorios y Control T\u00e9cnico","volume-title":"Resoluci\u00f3n 216\/2006. Buenos Aires: Servicio Nacional de Sanidad y Calidad Agroalimentaria","year":"2006"},{"key":"R27","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.trstmh.2008.10.004","article-title":"MLVA genotyping of human Brucella isolates from Peru","volume":"103","author":"Smits","year":"2009","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"R28","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1097\/01.md.0000165659.20988.25","article-title":"Brucellosis in San Diego: epidemiology and species related differences in acute clinical presentations","volume":"84","author":"Troy","year":"2005","journal-title":"Medicine"},{"key":"R29","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1111\/j.1469-0691.2007.01768.x","article-title":"Characterization of human outbreaks of brucellosis and sporadic cases by the use of hyper-variable octameric oligonucleotide fingerprint (HOOF) variable number tandem repeats","volume":"13","author":"Valdezate","year":"2007","journal-title":"Clin Microbiol Infect"},{"key":"R30","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.meegid.2009.07.001","article-title":"Current understanding of the genetic diversity of Brucella , an expanding genus of zoonotic pathogens","volume":"9","author":"Whatmore","year":"2009","journal-title":"Infect Genet Evol"},{"key":"R31","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1128\/JCM.02039-05","article-title":"Identification and characterization of variable number tandem repeat markers for typing of Brucella spp","volume":"44","author":"Whatmore","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R32","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.3201\/eid1108.041197","article-title":"Laboratory exposure to brucellae and implications for bioterrorism","volume":"11","author":"Yagupsky","year":"2005","journal-title":"Emerg Infect Dis"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.017525-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T14:43:40Z","timestamp":1601477020000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.017525-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,1]]},"references-count":32,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.017525-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,1]]}}}