{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:35:18Z","timestamp":1790519718981,"version":"4.1.0"},"reference-count":41,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:p><jats:italic>Streptococcus pneumoniae<\/jats:italic>, the aetiological agent of pneumonia and non-gonococcal urethritis, shares a high degree of DNA sequence identity with the viridans group of streptococci, particularly<jats:italic>Streptococcus mitis<\/jats:italic>and<jats:italic>Streptococcus oralis<\/jats:italic>. Although their clinical and pathological manifestations are different, discrimination between<jats:italic>S. pneumoniae<\/jats:italic>and its close viridans cocci relatives is still quite difficult. Suppression subtractive hybridization was performed to identify the genomic differences between<jats:italic>S. pneumoniae<\/jats:italic>and<jats:italic>S. mitis<\/jats:italic>. Thirty-four resulting<jats:italic>S. pneumoniae<\/jats:italic>-specific clones were examined by sequence determination and comparative DNA sequence analysis using<jats:sc>blast<\/jats:sc>.<jats:italic>S. pneumoniae<\/jats:italic>-specific primers were subsequently designed from one of the clonal DNA sequences containing the<jats:italic>cps<\/jats:italic>gene (coding for capsular polysaccharide biosynthesis). The primer specificities were evaluated using 49 viridans streptococci including 26<jats:italic>S. pneumoniae<\/jats:italic>, 54 other streptococci, 14<jats:italic>Lactococcus<\/jats:italic>species, 14<jats:italic>Enterococcus<\/jats:italic>species and three<jats:italic>Vagococcus<\/jats:italic>species, and compared with the specificities of previously described autolysin (<jats:italic>lytA<\/jats:italic>), pneumolysin (<jats:italic>ply<\/jats:italic>), Spn9802 and Spn9828 primers. The newly designed<jats:italic>cpsA<\/jats:italic>-specific primer set was highly specific to<jats:italic>S. pneumoniae<\/jats:italic>and was even better than the existing primers. These findings may help improve the rapid identification and differentiation of<jats:italic>S. pneumoniae<\/jats:italic>from closely related members of the viridans group streptococci.<\/jats:p>","DOI":"10.1099\/jmm.0.017798-0","type":"journal-article","created":{"date-parts":[[2010,7,9]],"date-time":"2010-07-09T02:26:33Z","timestamp":1278642393000},"page":"1146-1152","source":"Crossref","is-referenced-by-count":35,"title":["Identification of the cpsA gene as a specific marker for the discrimination of Streptococcus pneumoniae from viridans group streptococci"],"prefix":"10.1099","volume":"59","author":[{"given":"Hee Kuk","family":"Park","sequence":"first","affiliation":[{"name":"Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea"},{"name":"Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Wonkwang University College of Dentistry, Iksan 570-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang Won","family":"Yoon","sequence":"additional","affiliation":[{"name":"Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea"},{"name":"Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Wook","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyoung-Shik","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Wonkwang University College of Dentistry, Iksan 570-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joong-Ki","family":"Kook","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Chosun University College of Dentistry, Gwangju 501-825, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Chul","family":"Myung","sequence":"additional","affiliation":[{"name":"Department of Urology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea"},{"name":"Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of 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