{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:59:34Z","timestamp":1761580774672},"reference-count":27,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,1]]},"abstract":"<jats:p>This study was conducted to determine the types of <jats:italic>M. pneumoniae<\/jats:italic> prevalent in adults presenting with community-acquired pneumonia during an epidemic period, and to scrutinize a variable region of the RepMP4 element for the detection of P1 variants. All 23 clinical specimens PCR-positive for <jats:italic>M. pneumoniae<\/jats:italic> obtained in two hospitals in Santiago, Chile, from 2005 to 2006 were typed by a multiplex PCR directly and then the RepMP4 fragment of 18 specimens was sequenced. A predominance of <jats:italic>M. pneumoniae<\/jats:italic> type 2 was found, 18 (78.3\u200a%) specimens being grouped as type 2 and 5 (21.7\u200a%) as type 1. Co-infection of <jats:italic>M. pneumoniae<\/jats:italic> with other respiratory pathogens was found in 10\/23 (43.4\u200a%) patients, but their frequency was not related to the <jats:italic>M. pneumoniae<\/jats:italic> type. Sequence analysis revealed a single nucleotide polymorphism, a transition mutation, in 50\u200a% of amplicons belonging to type 1 and in 71.4\u200a% of amplicons of type 2. The nucleotide changes were synonymous in each P1 variant. In conclusion, during the 2005\u20132006 epidemic in Santiago, both types of <jats:italic>M. pneumoniae<\/jats:italic> circulated. Although the analysed area in the RepMP4 was small, we detected the existence of P1 variants in the two types of this organism.<\/jats:p>","DOI":"10.1099\/jmm.0.018333-0","type":"journal-article","created":{"date-parts":[[2010,5,7]],"date-time":"2010-05-07T01:11:40Z","timestamp":1273194700000},"page":"925-929","source":"Crossref","is-referenced-by-count":20,"title":["Identification of P1 types and variants of Mycoplasma pneumoniae during an epidemic in Chile"],"prefix":"10.1099","volume":"59","author":[{"given":"Mar\u00eda A.","family":"Mart\u00ednez","sequence":"first","affiliation":[{"name":"Programa de Microbiolog\u00eda, Facultad de Medicina, Universidad de Chile, Santiago de Chile, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universidad de Chile, Santiago de Chile, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enna","family":"Zunino","sequence":"additional","affiliation":[{"name":"Hospital de Infecciosos Dr Lucio C\u00f3rdova, Santiago de Chile, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian","family":"Luchsinger","sequence":"additional","affiliation":[{"name":"Programa de Virolog\u00eda, Facultad de Medicina, Universidad de Chile, Santiago de Chile, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Aguirre","sequence":"additional","affiliation":[{"name":"Centro de S\u00edntesis y An\u00e1lisis de Biomol\u00e9culas, Facultad de Medicina, Universidad de Chile, Santiago de Chile, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis F.","family":"Avenda\u00f1o","sequence":"additional","affiliation":[{"name":"Programa de Virolog\u00eda, Facultad de Medicina, Universidad de Chile, Santiago de Chile, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"4879","DOI":"10.1128\/JCM.41.10.4879-4882.2003","article-title":"Surveillance for respiratory syncytial virus in infants hospitalized for acute lower respiratory infection in Chile (1989 to 2000)","volume":"41","author":"Avenda\u00f1o","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"3631","DOI":"10.1128\/JCM.41.8.3631-3635.2003","article-title":"Comparative evaluation of real-time PCR assays for detection of the human metapneumovirus","volume":"41","author":"Cote","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1128\/JCM.38.3.965-970.2000","article-title":"Genotyping of Mycoplasma pneumoniae clinical isolates reveals eight P1 subtypes within two genomic groups","volume":"38","author":"Dorigo-Zetsma","year":"2000","journal-title":"J Clin Microbiol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"5612","DOI":"10.1128\/IAI.69.9.5612-5618.2001","article-title":"Mycoplasma pneumoniae P1 type 1- and type 2-specific sequences within the P1 cytadhesin gene of individual strains","volume":"69","author":"Dorigo-Zetsma","year":"2001","journal-title":"Infect Immun"},{"key":"R5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.ijmm.2004.06.020","article-title":"Preference, adaptation and survival of Mycoplasma pneumoniae subtypes in an animal model","volume":"294","author":"Dumke","year":"2004","journal-title":"Int J Med Microbiol"},{"key":"R6","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1128\/JCM.00495-06","article-title":"Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples","volume":"44","author":"Dumke","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1038\/298765a0","article-title":"Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody","volume":"298","author":"Feldner","year":"1982","journal-title":"Nature"},{"key":"R8","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1093\/infdis\/139.6.681","article-title":"Long-term epidemiology of infections with Mycoplasma pneumoniae","volume":"139","author":"Foy","year":"1979","journal-title":"J Infect Dis"},{"key":"R9","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/BF01586183","article-title":"Are outbreaks and sporadic respiratory infections by Mycoplasma pneumoniae due to two distinct subtypes?","volume":"15","author":"Jacobs","year":"1996","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"R10","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1378\/chest.08-0888","article-title":"Viral infection in adults hospitalized with community-acquired pneumonia. 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