{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:46:12Z","timestamp":1788407172848,"version":"build-2803163510"},"reference-count":18,"publisher":"Microbiology Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Campylobacter jejuni<\/jats:italic> is a major causative agent of diarrhoeal disease worldwide in the human population. In contrast, heavy colonization of poultry typically does not lead to disease and colonized chickens are a major source of <jats:italic>Campylobacter<\/jats:italic> infections in humans. Previously, we have shown that chicken (but not human) intestinal mucus inhibits <jats:italic>C. jejuni<\/jats:italic> internalization. In this study, we test the hypothesis that chicken mucin, the main component of mucus, is responsible for this inhibition of <jats:italic>C. jejuni<\/jats:italic> virulence. Purified chicken intestinal mucin attenuated <jats:italic>C. jejuni<\/jats:italic> binding and internalization into HCT-8 cells depending on the site of origin of the mucin (large intestine&gt;small intestine&gt;caecum). <jats:italic>C. jejuni<\/jats:italic> invasion of HCT-8 cells was preferentially inhibited compared to bacterial binding to cells. Exposure of the mucin to sodium metaperiodate recovered bacterial invasion levels, suggesting a glycan-mediated effect. However, fucosidase or sialidase pre-treatment of mucin failed to abrogate the inhibition of <jats:italic>C. jejuni<\/jats:italic> pathogenicity. In conclusion, differences in the composition of chicken and human intestinal mucin may contribute to the differential outcome of <jats:italic>Campylobacter<\/jats:italic> infection of these hosts.<\/jats:p>","DOI":"10.1099\/jmm.0.019315-0","type":"journal-article","created":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T01:12:05Z","timestamp":1273799525000},"page":"898-903","source":"Crossref","is-referenced-by-count":53,"title":["Purified chicken intestinal mucin attenuates Campylobacter jejuni pathogenicity in vitro"],"prefix":"10.1099","volume":"59","author":[{"given":"Abofu","family":"Alemka","sequence":"first","affiliation":[{"name":"The Children's Research Centre, Our Lady's Childrens' Hospital, Crumlin, Dublin 12, Ireland"},{"name":"School of Medicine and Medical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Whelan","sequence":"additional","affiliation":[{"name":"School of Medicine and Medical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan","family":"Gough","sequence":"additional","affiliation":[{"name":"School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marguerite","family":"Clyne","sequence":"additional","affiliation":[{"name":"The Children's Research Centre, Our Lady's Childrens' Hospital, Crumlin, Dublin 12, Ireland"},{"name":"School of Medicine and Medical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary 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