{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:46:58Z","timestamp":1771008418809,"version":"3.50.1"},"reference-count":38,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:p>This study focused on changes in selected parameters of humoral and cellular immunity following vaccination of mice with unique<jats:italic>Vibrio cholerae<\/jats:italic>LPS\u2013protein-complexed conjugates. The<jats:italic>V. cholerae<\/jats:italic>detoxified LPS (dLPS)-derived antigenic structures O-specific polysaccharide (O-SP) and de-<jats:italic>O<\/jats:italic>-acylated LPS (DeOAc-LPS) were used to prepare glycoconjugates by linking both dLPSs to glucan, the immunomodulating matrix, and then to BSA carrier. Animals were given a primary vaccination and boosted at 2-week intervals with a dose of 4.5\u2005\u03bcg saccharide antigen. The last boost was given either subcutaneously or intraperitoneally (i.p.) to compare the boosting effect and to optimize the effective immunization route. Both conjugates (O-SP\u2013BSA and DeOAc-LPS\u2013BSA) induced significant levels of antigen-specific Ig isotypes, especially IgG and IgM. The i.p. booster route was more effective. A T helper 1 response was achieved only by immunization with O-SP\u2013BSA conjugate administered i.p. Significant acceleration of phagocytic capacity and respiratory burst of neutrophils was demonstrated by both immunogenic formulations. Activation of T- and B-cell adaptive immunities was exhibited as specific changes in CD3\u200a:\u200aCD19 and CD4\u200a:\u200aCD8 ratios, B-cell low-affinity Fc<jats:italic>\u03b3<\/jats:italic>II and III receptor expression and induction of CD45R antigen.<\/jats:p>","DOI":"10.1099\/jmm.0.020875-0","type":"journal-article","created":{"date-parts":[[2010,8,20]],"date-time":"2010-08-20T02:25:20Z","timestamp":1282271120000},"page":"1440-1448","source":"Crossref","is-referenced-by-count":9,"title":["Immunological efficacy of glycoconjugates derived from Vibrio cholerae O1 serotype Ogawa detoxified LPS in mice"],"prefix":"10.1099","volume":"59","author":[{"given":"Ema","family":"Paulovi\u010dov\u00e1","sequence":"first","affiliation":[{"name":"Slovak Academy of Sciences, Institute of Chemistry, Centre of Excellence GLYCOMED, Dubravska cesta 9, 84236 Bratislava, 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