{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:34:23Z","timestamp":1784252063041,"version":"3.55.0"},"reference-count":46,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:p>Leptospirosis is a worldwide zoonosis caused by pathogenic <jats:italic>Leptospira.<\/jats:italic> The whole-genome sequence of <jats:italic>Leptospira interrogans<\/jats:italic> serovar Copenhageni together with bioinformatic tools allow us to search for novel antigen candidates suitable for improved vaccines against leptospirosis. This study focused on three genes encoding conserved hypothetical proteins predicted to be exported to the outer membrane. The genes were amplified by PCR from six predominant pathogenic serovars in Brazil. The genes were cloned and expressed in <jats:italic>Escherichia coli<\/jats:italic> strain BL21-SI using the expression vector pDEST17. The recombinant proteins tagged with N-terminal 6\u00d7His were purified by metal-charged chromatography. The proteins were recognized by antibodies present in sera from hamsters that were experimentally infected. Immunization of hamsters followed by challenge with a lethal dose of a virulent strain of <jats:italic>Leptospira<\/jats:italic> showed that the recombinant protein rLIC12730 afforded statistically significant protection to animals (44\u200a%), followed by rLIC10494 (40\u200a%) and rLIC12922 (30\u200a%). Immunization with these proteins produced an increase in antibody titres during subsequent boosters, suggesting the involvement of a T-helper 2 response. Although more studies are needed, these data suggest that rLIC12730 and rLIC10494 are promising candidates for a multivalent vaccine for the prevention of leptospirosis.<\/jats:p>","DOI":"10.1099\/jmm.0.021485-0","type":"journal-article","created":{"date-parts":[[2010,6,18]],"date-time":"2010-06-18T02:11:23Z","timestamp":1276827083000},"page":"1005-1015","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of leptospiral proteins that afford partial protection in hamsters against lethal challenge with Leptospira interrogans"],"prefix":"10.1099","volume":"59","author":[{"given":"Marina V.","family":"Atzingen","sequence":"first","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amane P.","family":"Gon\u00e7ales","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenaide M.","family":"de Morais","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo R.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical, Departamento de Patologia, Faculdade de Medicina, USP, Avenida Dr En\u00e9as Carvalho de Aguiar 470, 05403-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thales","family":"De Brito","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical, Departamento de Patologia, Faculdade de Medicina, USP, Avenida Dr En\u00e9as Carvalho de Aguiar 470, 05403-000 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr Orlando Marques de Paiva 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L. 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