{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:15:17Z","timestamp":1791288917661,"version":"4.3.0"},"reference-count":29,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"\n Pandoraea<\/jats:italic> species have emerged as opportunistic pathogens among cystic fibrosis (CF) and non-CF patients. Pandoraea<\/jats:italic> \n pulmonicola<\/jats:italic> is the predominant Pandoraea<\/jats:italic> species among Irish CF patients. The objective of this study was to investigate the pathogenicity and potential mechanisms of virulence of Irish P. pulmonicola<\/jats:italic> isolates and strains from other Pandoraea<\/jats:italic> species. Three patients from whom the P. pulmonicola<\/jats:italic> isolates were isolated have since died. The in vivo<\/jats:italic> virulence of these and other Pandoraea<\/jats:italic> strains was examined by determining the ability to kill Galleria mellonella<\/jats:italic> larvae. The P. pulmonicola<\/jats:italic> strains generally were the most virulent of the species tested, with three showing a comparable or greater level of virulence in vivo<\/jats:italic> relative to another CF pathogen, Burkholderia cenocepacia<\/jats:italic>, whilst strains from two other species, Pandoraea<\/jats:italic> \n apista<\/jats:italic> and Pandoraea<\/jats:italic> \n pnomenusa<\/jats:italic>, were considerably less virulent. For all Pandoraea<\/jats:italic> species, whole cells were required for larval killing, as cell-free supernatants had little effect on larval survival. Overall, invasive Pandoraea<\/jats:italic> strains showed comparable invasion of two independent lung epithelial cell lines, irrespective of whether they had a CF phenotype. Pandoraea<\/jats:italic> strains were also capable of translocation across polarized lung epithelial cell monolayers. Although protease secretion was a common characteristic across the genus, it is unlikely to be involved in pathogenesis. In conclusion, whilst multiple mechanisms of pathogenicity may exist across the genus Pandoraea<\/jats:italic>, it appears that lung cell invasion and translocation contribute to the virulence of P. pulmonicola<\/jats:italic> strains.<\/jats:p>","DOI":"10.1099\/jmm.0.022657-0","type":"journal-article","created":{"date-parts":[[2010,12,3]],"date-time":"2010-12-03T03:16:04Z","timestamp":1291346164000},"page":"289-299","source":"Crossref","is-referenced-by-count":49,"title":["Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells"],"prefix":"10.1099","volume":"60","author":[{"given":"Anne","family":"Costello","sequence":"first","affiliation":[{"name":"Centre of Applied Science for Health, ITT Dublin, Tallaght, Dublin 24, Ireland"},{"name":"Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Herbert","sequence":"additional","affiliation":[{"name":"Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Fabunmi","sequence":"additional","affiliation":[{"name":"Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Schaffer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, St Vincent's University Hospital, Elm Park, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Kavanagh","sequence":"additional","affiliation":[{"name":"Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma M.","family":"Caraher","sequence":"additional","affiliation":[{"name":"Centre of Applied Science for Health, ITT Dublin, Tallaght, Dublin 24, Ireland"},{"name":"Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1ire","family":"Callaghan","sequence":"additional","affiliation":[{"name":"Centre of Applied Science for Health, ITT Dublin, Tallaght, Dublin 24, Ireland"},{"name":"Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siobh\u00e1n","family":"McClean","sequence":"additional","affiliation":[{"name":"Centre of Applied Science for Health, ITT Dublin, Tallaght, Dublin 24, Ireland"},{"name":"Centre of Microbial Host Interactions, ITT Dublin, Tallaght, Dublin 24, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1128\/JCM.44.3.833-836.2006","article-title":"Chronic colonization with Pandoraea apista in cystic fibrosis patients determined by repetitive-element-sequence PCR","volume":"44","author":"Atkinson","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1099\/jmm.0.47544-0","article-title":"Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells","volume":"57","author":"Caraher","year":"2008","journal-title":"J Med Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1128\/JCM.39.5.1819-1826.2001","article-title":"Assignment of CDC weak oxidizer group 2 (WO-2) to the genus Pandoraea and characterization of three new Pandoraea genomospecies","volume":"39","author":"Daneshvar","year":"2001","journal-title":"J Clin Microbiol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.micpath.2006.07.005","article-title":"Differences in invasion and translocation of Burkholderia cepacia complex species in polarised lung epithelial cells in vitro","volume":"41","author":"Duff","year":"2006","journal-title":"Microb Pathog"},{"key":"R5","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1128\/IAI.01581-06","article-title":"Burkholderia cenocepacia requires a periplasmic HtrA protease for growth under thermal and osmotic stress and for survival in vivo","volume":"75","author":"Flannagan","year":"2007","journal-title":"Infect Immun"},{"key":"R6","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ejpb.2005.02.010","article-title":"Human respiratory epithelial cell culture for drug delivery applications","volume":"60","author":"Forbes","year":"2005","journal-title":"Eur J Pharm Biopharm"},{"key":"R7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-5173(00)00452-X","article-title":"Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery","volume":"208","author":"Foster","year":"2000","journal-title":"Int J Pharm"},{"key":"R8","doi-asserted-by":"crossref","first-page":"8247","DOI":"10.1128\/JB.187.24.8247-8255.2005","article-title":"Distribution and expression of the ZmpA metalloprotease in the Burkholderia cepacia complex","volume":"187","author":"Gingues","year":"2005","journal-title":"J Bacteriol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"3843","DOI":"10.1128\/JB.182.13.3843-3845.2000","article-title":"Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects","volume":"182","author":"Jander","year":"2000","journal-title":"J Bacteriol"},{"key":"R10","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1097\/01.inf.0000136857.74561.3c","article-title":"Pandoraea bacteremia in a cystic fibrosis patient with associated systemic illness","volume":"23","author":"Johnson","year":"2004","journal-title":"Pediatr Infect Dis J"},{"key":"R11","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1002\/ppul.10383","article-title":"Epidemic spread of Pandoraea apista , a new pathogen causing severe lung disease in cystic fibrosis patients","volume":"36","author":"J\u00f8rgensen","year":"2003","journal-title":"Pediatr Pulmonol"},{"key":"R12","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.femsre.2003.09.002","article-title":"Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens","volume":"28","author":"Kavanagh","year":"2004","journal-title":"FEMS Microbiol Rev"},{"key":"R13","doi-asserted-by":"crossref","first-page":"7107","DOI":"10.1128\/IAI.73.11.7107-7112.2005","article-title":"Disruption of tight junctions during traversal of the respiratory epithelium by Burkholderia cenocepacia","volume":"73","author":"Kim","year":"2005","journal-title":"Infect Immun"},{"key":"R14","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1128\/IAI.00297-06","article-title":"Burkholderia cenocepacia ZmpB is a broad-specificity zinc metalloprotease involved in virulence","volume":"74","author":"Kooi","year":"2006","journal-title":"Infect Immun"},{"key":"R15","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1055\/s-2004-815664","article-title":"Burkholderia and emerging pathogens in cystic fibrosis","volume":"24","author":"LiPuma","year":"2003","journal-title":"Semin Respir Crit Care Med"},{"key":"R16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/JCM.19.2.291-293.1984","article-title":"Characterization of Pseudomonas cepacia isolates from patients with cystic fibrosis","volume":"19","author":"McKevitt","year":"1984","journal-title":"J Clin Microbiol"},{"key":"R17","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1128\/JCM.00855-08","article-title":"Misidentification of Pandoraea sputorum isolated from sputum of a patient with cystic fibrosis and review of Pandoraea species infections in transplant patients","volume":"46","author":"Pimentel","year":"2008","journal-title":"J Clin Microbiol"},{"key":"R18","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1023\/B:MYCO.0000038434.55764.16","article-title":"Correlation between gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella","volume":"158","author":"Reeves","year":"2004","journal-title":"Mycopathologia"},{"key":"R19","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1128\/AAC.50.4.1330-1335.2006","article-title":"Novel carbapenem-hydrolyzing oxacillinase OXA-62 from Pandoraea pnomenusa","volume":"50","author":"Schneider","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"R20","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1128\/IAI.01249-07","article-title":"Development of Galleria mellonella as an alternative infection model for the Burkholderia cepacia complex","volume":"76","author":"Seed","year":"2008","journal-title":"Infect Immun"},{"key":"R21","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1128\/AAC.01166-08","article-title":"Experimental bacteriophage therapy increases survival of Galleria mellonella larvae infected with clinically relevant strains of the Burkholderia cepacia complex","volume":"53","author":"Seed","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"R22","first-page":"L493","article-title":"Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl\u2212 secretion","volume":"266","author":"Shen","year":"1994","journal-title":"Am J Physiol"},{"key":"R23","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1128\/JCM.41.5.2255-2257.2003","article-title":"Sepsis, multiple organ failure, and death due to Pandoraea pnomenusa infection after lung transplantation","volume":"41","author":"Stryjewski","year":"2003","journal-title":"J Clin Microbiol"},{"key":"R24","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1128\/JB.149.3.1027-1033.1982","article-title":"Subcellular distribution of various proteases in Escherichia coli","volume":"149","author":"Swamy","year":"1982","journal-title":"J Bacteriol"},{"key":"R25","doi-asserted-by":"crossref","first-page":"4102","DOI":"10.1128\/IAI.00398-09","article-title":"Identification of specific and universal virulence factors in Burkholderia cenocepacia strains by using multiple infection hosts","volume":"77","author":"Uehlinger","year":"2009","journal-title":"Infect Immun"},{"key":"R26","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1128\/JCM.38.3.1042-1047.2000","article-title":"Identification and population structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia genomovar IV","volume":"38","author":"Vandamme","year":"2000","journal-title":"J Clin Microbiol"},{"key":"R27","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1086\/502558","article-title":"Isolation of infectious cystic fibrosis patients: results of a systematic review","volume":"26","author":"Vonberg","year":"2005","journal-title":"Infect Control Hosp Epidemiol"},{"key":"R28","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1099\/jmm.0.46199-0","article-title":"Burkholderia cenocepacia utilizes ferritin as an iron source","volume":"55","author":"Whitby","year":"2006","journal-title":"J Med Microbiol"},{"key":"R29","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1128\/JCM.02273-07","article-title":"Differential mucoid exopolysaccharide production by members of the Burkholderia cepacia complex","volume":"46","author":"Zlosnik","year":"2008","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.022657-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T18:30:45Z","timestamp":1601577045000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.022657-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,1]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.022657-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,1]]}}}