{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T08:56:48Z","timestamp":1767085008131},"reference-count":21,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p>\n                  <jats:italic>Laribacter hongkongensis<\/jats:italic> is a potential emerging pathogen, associated with community-acquired diarrhoea. For epidemiological purposes, different molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing, have been developed for this pathogen. However, these methods require specialized equipment and costly reagents. More importantly, they are labour-intensive and time-consuming, which is not really suitable for foodborne disease outbreak investigations. In this study, we developed a rapid and reliable method using 22-mer primers specific for the enterobacterial repetitive intergenic consensus sequence (ERIC). PFGE was used for comparison, to evaluate this method. A total of 81 isolates of <jats:italic>L. hongkongensis<\/jats:italic> were examined: 79 isolates recovered from food of diverse origins and two strains derived from patients with <jats:italic>L. hongkongensis<\/jats:italic>-associated infection. Typing patterns and clustering analysis indicated that the 81 <jats:italic>L. hongkongensis<\/jats:italic> isolates were grouped into 21 and 13 genotypes by ERIC-PCR and PFGE, respectively. ERIC-PCR was found as reproducible as PFGE. A high percentage (70.4\u200a%) of isolates yielded distinguishable ERIC-PCR patterns, which were concordant with the results from PFGE. These results suggest that ERIC-PCR is valuable for use in the epidemiological investigation of <jats:italic>L. hongkongensis<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.049858-0","type":"journal-article","created":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T02:13:52Z","timestamp":1358475232000},"page":"701-707","source":"Crossref","is-referenced-by-count":10,"title":["Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers\u2019 diarrhoea"],"prefix":"10.1099","volume":"62","author":[{"given":"Jia-Li","family":"Feng","sequence":"first","affiliation":[{"name":"Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, Southern Medical University, Guangzhou, Guangdong Province 510515, China"},{"name":"Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Yan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Bing","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Light Industry and Food Science, South China University of Technology Guangzhou, 510000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang-Feng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Public Health and Tropical 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Institutes, Southern Medical University, Guangzhou, Guangdong Province 510515, China"},{"name":"Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2010.10.014"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2012.04.018"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1080\/00365540110076787"},{"key":"r4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1099\/00207713-51-1-3","article-title":"Genomic approaches to typing, taxonomy and evolution of bacterial isolates","volume":"51","author":"G\u00fcrtler","year":"2001","journal-title":"Int J Syst Evol 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