{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:08:08Z","timestamp":1762250888758},"reference-count":33,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,4,1]]},"abstract":"<jats:p>A detection system for <jats:italic>Legionella<\/jats:italic> DNA in blood samples based on the PCR was developed and evaluated in A\/J mice with experimentally induced <jats:italic>Legionella<\/jats:italic> pneumonia. Primers were designed to amplify a 106 bp DNA fragment of the 16S rRNA gene specific to <jats:italic>Legionella<\/jats:italic> species. The PCR system could detect clinically relevant <jats:italic>Legionella<\/jats:italic> species including <jats:italic>Legionella pneumophila<\/jats:italic>, <jats:italic>Legionella micdadei<\/jats:italic>, <jats:italic>Legionella bozemanae<\/jats:italic>, <jats:italic>Legionella dumoffii<\/jats:italic>, <jats:italic>Legionella longbeachae<\/jats:italic>, <jats:italic>Legionella gormanii<\/jats:italic> and <jats:italic>Legionella jordanis<\/jats:italic>. The sensitivity of the PCR system was 20 fg extracted DNA. In the mouse model, the blood PCR was compared with results obtained by PCR on bronchoalveolar lavage fluid (BALF) samples, cultures of blood and BALF and detection of <jats:italic>Legionella<\/jats:italic> urinary antigen. Blood PCR was positive until 8 days after infection, while BALF PCR became negative on day 4. These results indicate that PCR using blood samples may be a useful, convenient and non-invasive method for the diagnosis of <jats:italic>Legionella<\/jats:italic> pneumonia.<\/jats:p>","DOI":"10.1099\/jmm.0.04999-0","type":"journal-article","created":{"date-parts":[[2003,4,3]],"date-time":"2003-04-03T22:52:16Z","timestamp":1049410336000},"page":"325-329","source":"Crossref","is-referenced-by-count":8,"title":["Detection of Legionella DNA by PCR of whole-blood samples in a mouse model"],"prefix":"10.1099","volume":"52","author":[{"given":"S.","family":"Aoki","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Hirakata","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Izumikawa","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yanagihara","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Tomono","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Tashiro","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kohno","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kamihira","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine1 and Second Department of Internal Medicine2, Nagasaki University School of Medicine, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"H1-8","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1007\/s150100050031","article-title":"Diagnosis by polymerase chain reaction of pneumonia caused by Legionella pneumophila in an immunocompetent child","volume":"27","author":"Aebischer","year":"1999","journal-title":"Infection"},{"key":"H2-8","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1128\/JCM.38.7.2763-2765.2000","article-title":"Evaluation of the Binax and Biotest urinary antigen kits for detection of Legionnaires\u2019 disease due to multiple serogroups and species of Legionella","volume":"38","author":"Benson","year":"2000","journal-title":"J Clin Microbiol"},{"key":"H3-8","first-page":"84","article-title":"Legionnaires\u2019 disease: clinical, epidemiological, and public health perspectives","volume":"13","author":"Breiman","year":"1998","journal-title":"Semin Respir Infect"},{"key":"H4-8","first-page":"1537","article-title":"Replicative Legionella pneumophila lung infection in intratracheally inoculated A\/J mice. 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